Skip to main content
SpringerLink
Log in

Inducible transcription of the dnaA gene from Streptomyces lividans 66

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Abstract

The dnaA gene of Streptomyces lividans was cloned using the Escherichia coli medium-copy-number vector pSU18 and E. coli strain TC1963, which can bypass the requirement for the DnaA protein. Its regulatory region was subcloned in the Streptomyces probe vector pIJ4083. Primer extension and S1 mapping studies allowed the identification of a class I Streptomyces promotor (P2). An additional, previously unknown promoter type (P1) was found by S1 mapping. The presence of two DnaA box motifs between P1 and P2 suggests that the transcriptions of the S. lividans dnaA gene is autoregulated by its gene product. It was shown that the transcription of the dnaA gene is significantly induced by mitomycin C, an agent known to inhibit DNA replication. The data suggest that, as in E. coli, one of the regulatory mechanisms governing the transcription of the dnaA gene in S. lividans is probably related to the SOS response network.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Andrup L, Atlung T, Ogasawara N, Yoshikawa H, Hansen FG (1988) Interaction of the Bacillus subtilis DnaA-like protein with the Escherichia coli DnaA protein. J Bacteriol 170:1333–1338

    Google Scholar 

  • Atlung T, Clausen ES, Hansen FG (1985) Autoregulation of the dnaA gene of Escherichia coli K12. Mol Gen Genet 200:442–450

    Google Scholar 

  • Atlung T, Løbner-Olesen A, Hansen FG (1987) Overproduction of DnaA protein stimulates initiating of chromosome and minichromosome replication in E. coli. Mol Gen Genet 206:51–59

    Google Scholar 

  • Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1990) Current protocols in molecular biology. Greene Publishing Associates/Wiley-Interscience, New York, pp 481–482

    Google Scholar 

  • van den Berg EA, Geerse RH, Mamelink J, Bovenberg RAL, Magnee FA, van de Putte P (1985) Analysis of regulatory sequences upstream the E. coli norB gene: involvement of the DnaA protein. Nucleic Acids Res 13:1829–1840

    Google Scholar 

  • Birnboim HC (1983) A rapid alkaline extraction method for isolation of plasmid DNA. Methods Enzymol 100: 243–255

    Google Scholar 

  • Borowy-Boroski H, Lipman R, Chowdary D, Tomasz M (1990) Duplex oligodeoxyribonucleotides cross-linked by mitomycin C at a single site: synthesis, properties, and cross-link reversibility. Biochemistry 29:2992–2999

    Google Scholar 

  • Bramhill D, Kornberg A (1988) A model for initiation at origins of DNA replication. Cell 54:915–918

    Google Scholar 

  • Braun RE, O'Day K, Wright A (1985) Autoregulation of the DNA replication gene dnaA in E. coli K12. Cell 40:159–169

    Google Scholar 

  • Calcutt MJ, Schmidt FJ (1992) Conserved gene arrangement in the origin region of the Streptomyces coelicolor chromosome. J Bacteriol 174:3220–3226

    Google Scholar 

  • Chiaramello AE, Zyskind JW (1990) Coupling of DNA replication to growth rate in Escherichia coli: a possible role for guanosine tetraphosphate. J Bacteriol 172:2013–2019

    Google Scholar 

  • Clayton TM, Bibb MJ (1989) Streptomyces promoter-probe plasmids that utilise the xylE gene of Pseudomonas putida. Nucleic Acids Res 18:1077

    Google Scholar 

  • Delic I, Robbins P, Westpheling J (1992) Direct repeat sequences are implicated in the regulation of two Streptomyces chitinase promoters that are subject to carbon cabolite control. Proc Natl Acad Sci USA 89:1885–1889

    Google Scholar 

  • Ellison KS, Dogliotti E, Connors TD, Basu AK, Essigmann JM (1989) Site-specific mutagenesis by O 6-alkylguanines located in the chromosomes of mammalian cells: Influence of the mammalian O 6-alkylguanine-DNA alkylguanine-DNA alkyltransferase. Proc Natl Acad Sci USA 86:8620–8624

    Google Scholar 

  • Fornwald JA, Schmidt FJ, Adams CW, Rosenberg M, Brawner ME (1987) Two promoters, one inducible and one constitutive, control transcription of the Streptomyces lividans galactose operon. Proc Natl Acad Sci USA 84:2130–2134

    Google Scholar 

  • Fujita MQ, Yoshikawa H, Ogasawara N (1990) Structure of the dnaA region of Micrococcus luteus: conservation and variations among eubacteria. Gene 93:73–78

    Google Scholar 

  • Fujita MQ, Yoshikawa H, Ogasawara N (1992) Structure of the dnaA and DnaA-box region in the Mycoplasma capricolum chromosome: conservation and variation in the course of evolution. Gene 110:17–23

    Google Scholar 

  • Hansen FG, Hansen EB, Atlung T (1982) The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34 of E. coli. EMBO J 1:1043–1048

    Google Scholar 

  • Hansen FG, Atlung T, Braun RE, Wright A, Hughes P, Kohiyama M (1991) Initiator (DnaA) protein concentration as a function of growth rate in Escherichia coli and Salmonella typhimurium. J Bacteriol 173:5194–5199

    Google Scholar 

  • Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM, Schrempf H (1985) Genetic manipulation of Streptomyces: a laboratory manual. The John Innes Institute, Norwich, UK

    Google Scholar 

  • Horii M, Ishizaki T, Paik S-Y, Manome T, Murooka Y (1990) An operon containing the genes for cholesterol oxidase and a cytochrome P-450-like protein from a Streptomyces sp. J Bacteriol 172:3644–3653

    Google Scholar 

  • Ingmer H, Atlung T (1991) Expression and regulation of a dnaA homologue isolated from Pseudomonas putida. Mol Gen Genet 232:431–439

    Google Scholar 

  • Kaasch M, Kaasch J, Quiñones A (1989) Expression of the dnaA and dnaQ genes of E. coli is inducible by mitomycin C. Mol Gen Genet 219:187–192

    Google Scholar 

  • Kieser HM, Kieser T, Hopwood DA (1992) A combined genetic and physical map of the Streptomyces coelicolor A3(2) chromosome. J Bacteriol 174:5496–5507

    Google Scholar 

  • Kücherer C, Lother H, Kölling R, Schauzu M-A, Messer W (1986) Regulation of transcription of the chromosomal dnaA gene of Escherichia coli 205:115–121

    Google Scholar 

  • Kutzner HJ (1981) The family Streptomycetaceae. In: Starr MP, Stolp H, Trüper HG, Balows A, Schegel H (eds) The prokaryotes: a handbook on habitats, isolation and identification of bacteria. Springer-Verlag KG, Berlin, pp 2028–2090

    Google Scholar 

  • Løbner-Oleson A, Atlung T, Rasmussen KV (1987) Stability and replication control of Escherichia coli minichromosomes. J Bacteriol 169:2835–2842

    Google Scholar 

  • Masters M, Paterson T, Popplewll AG, Owen-Hughes T, Pringle JH, Begg KJ (1989) The effect of DnaA protein levels and the rate of initiation at oriC on transcription originating in the ftsQ and ftsA genes: In vivo experiments. Mol Gen Genet 216:475–483

    Google Scholar 

  • Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Moriya S, Atlung T, Hansen FG, Yoshikawa H, Ogasawara N (1992) Cloning of an autonomously replicating sequence (ars) from the Bacillus subtilis chromosome. Mol Microbiol 6:309–315

    Google Scholar 

  • Ogasawara N, Moriya S, Yoshikawa H (1991) Initiation of chromosome replication: structure and function of oriC and DnaA protein in eubacteria. Res Microbiol 142:851–859

    Google Scholar 

  • Quiñones A, Kaasch J, Kaasch M, Messer W (1989) Induction of dnaN and dnaQ gene expression in Escherichia coli by alkylation damage to DNA. EMBO J 8:587–593

    Google Scholar 

  • Quiñones A, Riterbock WR, Messer W (1991a) Expression of dnaA gene of Escherichia coli is inducible by DNA damage. Mol Gen Genet 227:9–16

    Google Scholar 

  • Quiñones A, Jüterbock WR, Messer W (1991b) DNA lesions that block DNA replication are responsible for the dnaA induction caused by DNA damage. Mol Gen Genet 231:81–87

    Google Scholar 

  • Sakakibara Y, Yuasa S (1982) Continuous synthesis of the dnaA gene product of Escherichia coli in the cell cycle. Mol Gen Genet 186:87–94

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Simonet JM, Schneider D, Volff JN, Dary A, Decaris B (1992) Genetic instability in Streptomyces ambofaciens: inducibility and associated genome plasticity. Gene 115:49–54

    Google Scholar 

  • Strohl WR (1992) Compilation and analysis of DNA sequences associated with apparent streptomycete promoters. Nucleic Acids Res 20:961–974

    Google Scholar 

  • Verma M, Moffat K, Egan B (1989) UV irradiation inhibits initiation of DNA replication from oriC in E. coli. Mol Gen Genet 216:446–454

    Google Scholar 

  • Wood WB (1966) Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. J Mol Bio 16:118

    Google Scholar 

  • Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC18 vectors. Gene 33:103–119

    Google Scholar 

  • Yee TW, Smith DW (1990) Pseudomonas1 chromosomal replication origins: a bacterial class distinct from Escherichia coli-type origins. Proc Natl Acad Sci USA 87:1278–1282

    Google Scholar 

  • Yoshikawa H, Ogasawara N (1991) Structure and function of DnaA and the DnaA-box in eubacteria: evolutionary relationships of bacterial replication origins. Mol Microbiol 5:2589–2597

    Google Scholar 

  • Zakrzewska-Czerwińska J, Schrempf H (1992) Characterization of an autonomously replicating region from the Streptomyces lividans chromosome. J Bacteriol 174:2688–2693

    Google Scholar 

  • Zotchev SB, Soldatowa LI, Orekhov AV, Schrempf H (1992) Characterization of a linear extrachromosomal DNA element (pBL1) isolated after interspecific mating between Streptomyces bambergiensis and Streptomyces lividans. Res Microbiol 143:839–846

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, Institute of Immunology and Experimental Therapy, Czerska 12, 53-114, Wrocław, Poland

    Jolanta Zakrzewska-Czerwińska

  2. FB Biologie/Chemie, Universität Osnabrück, Barbarastraße 11, 49069, Osnabrück, Germany

    Jolanta Zakrzewska-Czerwińska, Judith Nardmann & Hildgund Schrempf

Authors
  1. Jolanta Zakrzewska-Czerwińska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Judith Nardmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hildgund Schrempf
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Böhme

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zakrzewska-Czerwińska, J., Nardmann, J. & Schrempf, H. Inducible transcription of the dnaA gene from Streptomyces lividans 66. Molec. Gen. Genet. 242, 440–447 (1994). https://doi.org/10.1007/BF00281794

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00281794

Key words

Search

Navigation