Abstract
Three new glutathione transferase (GST) genes from the housefly Musca domestica are described. These genes, identified as MdGST-2, -3, and -4, were from cDNA clones obtained from a cDNA bank in phage λ. The bank was prepared using poly(A)+ RNA from a housefly that is highly resistant to organophosphate insecticides because of enhanced expression of multiple members of the glutathione transferase gene family. The DNA sequence of each is reported and has a complete open reading frame that specified an amino acid sequence similar to other dipteran glutathione transferases. Based on phylogenetic analysis, we can conclude that the insect glutathione transferase gene family falls into two groups, each of which evolves at a different rate, presumably due to differences in functional constraints. We show that MdGST-1 (and their homologues from Drosophila and Lucilia) evolve at a significantly slower rate than the other members of the gene family. Each housefly GST cDNA was inserted into a bacterial plasmid expression system and a glutathione transferase activity was expressed in Escherichia coli. The transcription pattern of each of these glutathione transferases was examined in a variety of different housefly strains that are known to differ in their resistance to organophosphate insecticides due to different patterns of glutathione transferase expression. We found that the level of transcription for two of our clones was positively correlated with the level of organophosphate resistance.
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References
Arca P, Garcia P, Hardisson C, Suarez JE (1990) Purification and study of a bacterial glutathione S-transferase. FEBS Lett 263:77–79
Clark AG, Dauterman WC (1982) Characterization by affinity chromatography of glutathione S-transferases from different strains of housefly. Pest Biochem Physiol 17:307–314
Clark AG, Shamaan GNA, Dauterman WC, Hayaoka, T (1984) Characterization of multiple glutathione S-transferases from the housefly, Musca domestica. Pest Biochem Physiol 22:51–59
Fournier D, Bride JM, Poirie M, Berge JB, Plapp FW Jr (1992) Insect glutathione transferases. Characteristics of the major forms from houseflies susceptible and resistant to insecticides. J Biol Chem 267:1840–5
Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139
Mannervik B (1985) Adv Enzymol 57:357–413
Oppenoorth F, Rupes V, El Bashir S, Houx N, Voermans S (1972) Glutathione-dependent degradation of parathion and its significance for resistance in the housefly. Pest Biochem Physiol 2:262–277
Oppenoorth F, Smissaert H, Welling W, van der Pas L, Hitman, K (1977) Insensitive acetylcholinesterase, high glutathione Stransferase and hydrolytic activity as resistance factors in a TCVP-resistant strain of housefly. Pest Biochem Physiol 7:34–47
Plapp FW (1984) Genetic basis of insecticide resistance in the housefly. Pest Biochem Physiol 22:194–201
Sambrook J, Fritsch, EF, Maniatis T (1989) Molecular cloning 2nd edn Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Tabor S, Richardson CC (1985) A bacteriophage T7 polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA 82:1074–8
Toung YP, Tu CP (1992) Drosophila glutathione S-transferases have sequence homology to the stringent starvation protein of Escherichia coli. Biochem Biophys Res Comm 182:355–360
Toung YP, Hsieh TS, Tu CP (1990) Drosophila glutathione S-transferase 1-1 shares a region of sequence homology with the maize glutathione S-transferase III. Proc Natl Acad Sci USA 87:31–35
Toung YP, Hsieh TS, Tu CP (1993) The glutathione S-transferase D genes. A divergently organized, intronless gene family in D. melanogaster. J. Biol Chem 268:9737–46
Wang JY, McCommas S, Syvanen M (1991) Molecular cloning of a glutthione S-transferase overproduced in an insecticide-resistant strain of the housefly (Musca domestica). Mol Gen Genet 227:260–266
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Communicated by D. J. Finnegan
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Syvanen, M., Zhou, Zh. & Wang, Jy. Glutathione transferase gene family from the housefly Musca domestica . Molec. Gen. Genet. 245, 25–31 (1994). https://doi.org/10.1007/BF00279747
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DOI: https://doi.org/10.1007/BF00279747