Summary
Experiments were performed in order to gain information about the primary process leading to the production of sister chromatid exchanges (SCEs). Radical-forming substances (hydroxylamine, hydrazine and the antituberculous drug isoniazid) were examined for their effectiveness in inducing SCEs. All three substances proved successful in the induction of SCEs in the V-79 cell line of the Chinese hamster. By simultaneous application of a sulfhydryl compound (cysteine), a reduction of the hydrazine-and isoniazid-induced SCEs was achieved. Isoniazid was additionally examined in the in vivo SCE-test. At concentrations of 2–100 mg/kg body weight, it does not increase the rate of SCEs in the bone marrow of the Chinese hamster.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allen, J. W., Shuler, C. F., Latt, S. A.: Bromodeoxyuridine tablet methodology for in vivo studies of DNA-synthesis. Somat. Cell Genet. 4, 393–405 (1978)
Asada, K., Kanematsu, S.: Reactivity of thiols with superoxide radicals. Agr. Biol. Chem. 40, 1891–1892 (1976)
Basler, A.: Analysis of chromosome aberrations in bone marrow cells of rats after treatment with isoniazid. Hum. Genet. 42, 26–27 (1978)
Bauchinger, M., Gebhart, E., Fonatsch, C., Schmid, E., Müller, W., Obe, G., Beek, B., Göbel, D., Radenbach, K. L.: Chromosome analyses in man in the course of chemoprophylaxis against tuberculosis and of antituberculosis chemotherapy with isoniazid. Hum. Genet. 42, 31–43 (1978)
Braun, L., Schuber, J., Schöneich, J.: On the mutagenicity of isoniazid. Biol. Zentralblatt 95, 423–436 (1976)
Brown, P. E.: Mechanism of action of aminothiol radio-protectors. Nature 28, 363–364 (1967)
Epplen, J. T., Siebers, J. W., Vogel, W.: DNA replication patterns of human chromosomes from fibroblasts and amniotic fluid cells revealed by a Giemsa staining technique. Cytogen. Cell. Genet. 15, 177–185 (1975)
Engel, W., Krone, W., Wolf, U.: Die Wirkung von Thioguanin, Hydroxylamin und 5-Bromdesoxyuridin auf menschliche Chromosomen in vitro. Mut. Res. 4, 353–368 (1967)
Freese, E.: Molecular mechanisms of mutations. In: Chemical mutagens (A. Hollaender, ed.), pp. 1–56. New York, London: Plenum Press 1971
Gebhart, E.: Die Wirkung von L-Cystein auf die Aberrationsauslösung in menschlichen Chromosomen. I. Dosis-Wirkung-Beziehungen bei Verwendung von Trenimon® als Aberrationsinduktor. Humangenetik 10, 115–126 (1970)
Gebhart, E.: L-Cystein als Protektor gegen die aberrationsauslösende Wirkung von 8-Hydroxylchinolinsulfat in menschlichen Leukozytenchromosomen. Mut. Res. 11, 261–262 (1971a)
Gebhart, E.: Die Wirkung von L-Cystein auf die Aberrationsauslösung in menschlichen Chromosomen durch chemische Mutagene. II. Zur Bedeutung des Zeitfaktors bei Verwendung von Trenimon® als Aberrationsinduktor. Humangenetik 11, 237–243 (1971b)
Kappauf, H.: Der Einfluß von Anticlastogenen auf den chemisch-induzierten Schwesterchromatidaustausch. Dissertation Med. Fakultät der Universität Erlangen-Nürnberg, 1978
Müller, D., Langauer, D., Grafe, A.: Nucleus anomaly test in chinese hamster and rat after treatment with isoniazid. Hum. Genet. 42, 9–14 (1978a)
Müller, D., Strasser, F. F., Röhrborn, G., Tränz, J., Grafe, A., Miltenburger, H. G., Träger, H., Pawlowikki, J. H., Nautsch, C., Okimoto, S., Frohberg, H., Schulze-Schenking, N.: Chromosome analysis of bone marrow in mammals after treatment with isoniazid. Hum. Genet. 42, 15–25 (1978b)
Perry, P., Evans, H. J.: Cytological detection of mutagencarcinogen exposure by sister chromatid exchange. Nature 258, 121–125 (1975)
Röhrborn, G., Miltenburger, H. G., Radenbach, K. L.: A correlated study of the cytogenetic effect of isoniazed (INH) on cell systems of mammals and man conducted by thirteen laboratories. Introductory remarks. Hum. Genet. 42, 3–7 (1978)
Rosin, N. P., Stich, H. F.: Inhibitory effect of reducing agents on N-acetoxy- and N-hydroxy-2-acetylamino-fluorene-induced mutagenesis. Cancer Research 38, 1307–1310 (1978)
Sasaki, N. S., Matsubara, S.: Free radical scavenging in protection of human lymphocytes against chromosome aberration formation by gamma-ray irradiation. Int. J. Radiat. Biol. 32, 439–445 (1977)
Schneider, E. L., Tice, R. R., Kram, D.: Bromodeoxyuridine differential chromatid staining technique: a new approach to examining sister chromatid exchange and cell replication kinetics. In: Methods in cell biology, Vol. XX (D. N. Prescott, ed.)., pp. 379–409. New York, San Francisco: Academic Press 1978
Speit, G., Vogel, W.: The effect on sister-chromatid exchanges of drugs and dyes by intercalation and photoactivation. Mut. Res. 59, 223–229 (1979)
Somers, C. E., Hsu, T. C.: Chromosome damage induced by Hydroxylamine in mammalian cells. P.N.A.S. 48, 937–943 (1962)
Wolff, S.: Sister chromatid exchange. Ann. Rev. Genet. 11, 183–201 (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Speit, G., Wick, C. & Wolf, M. Induction of sister chromatid exchanges by hydroxylamine, hydrazine and isoniazid and their inhibition by cysteine. Hum Genet 54, 155–158 (1980). https://doi.org/10.1007/BF00278964
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00278964