Summary
A modified thermal denaturation of human chromosomes in Hanks' solution at 91°C produces highly contrasted C and T bands. This improved double-banding system is a good tool in the analysis of ring chromosomes, for which R banding is often inadequate, as it is exemplified.
This is a preview of subscription content, log in via an institution to check access.
References
Arrighi, F. E., Hsu, T. C.: Localization of heterochromatin in human chromosomes Cytogenetics 10, 81–86 (1971)
Comings, D. E., Avelino, E., Okada, T. A., Wyandt, H. E.: The mechanism of C and G-banding of chromosomes. Exp. Cell Res. 77, 469–493 (1973)
Dutrillaux, B.: Nouveau système de marquage chromosomique: les bandes T. Chromosoma (Berl.) 41, 395–402 (1973)
Dutrillaux, B.: Sur la nature et l'origine des chromosomes humains. Monographie des Annales de Génétique. Paris: l'Expansion Scientifique 1975
Dutrillaux, B., Covic, M.: Etude de facteurs influençant la dénaturation thermique ménagée des chromosomes. Exp. Cell Res. 85, 143–153 (1974)
Moorhead, P. S., Nowell, P. C., Mellman, W. J., Batipps, D. M., Hungerford, D. A.: Chromosome preparations of leucocytes cultured from human peripheral blood. Exp. Cell Res. 20, 613–616 (1960)
Paris Conference, 1971: Standardization in human cytogenetics: Birth defects. Original article series VIII,7. New York: National Foundation 1972
Scheres, J. M. J. C.: Production of C and T bands in human chromosomes after heat treatment at high pH and staining with “Stains All”. Humangenetik 23, 311–314 (1974)
Author information
Authors and Affiliations
Additional information
Service Prof. J. Battin
Rights and permissions
About this article
Cite this article
Chamla, Y., Ruffié, M. Production of C and T bands in human mitotic chromosomes after heat treatment. Hum. Genet. 34, 213–216 (1976). https://doi.org/10.1007/BF00278891
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00278891