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Toxicity of antibiotics on cultured human skin fibroblasts

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Summary

Human diploid fibroblasts cultured in Dulbecco's Modified Eagle's medium (DME) were exposed to different concentrations of 15 antibiotics to determine the limiting toxic concentration. The number of cells surviving after antibiotic treatment was given as the index of toxicity. No visible chromosomal damage could be detected when half the maximal toxic concentration was applied. The maximum limiting concentration was found to be the same for both the preconfluent and postconfluent phases.

Zusammenfassung

15 Antibiotica wurden in verschiedenen Konzentrationen menschlichen diploiden Fibroblasten, gezüchtet in Dulbecco's Modified Eagle's medium (DME), zugesetzt. Die Grenze der toxischen Konzentration wurde für jedes Antibioticum durch Zellzählungen in unabhängigen Versuchen bestimmt. Die für den antimikrobiellen Einsatz verwendbaren Maximalkonzentrationen wurden angegeben als die Hälfte der minimalen toxischen Konzentrationen. Keines der getesteten Antibiotica führte bei diesen Konzentrationen zu chromosomalen Aberrationen. Die toxische Grenze war jeweils gleich für Zellen der präkonfluenten und postkonfluenten Phase.

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References

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Authors and Affiliations

  1. Institut für Humangenetik, Universität Hamburg, Hamburg, Germany

    W. Byarugaba, H. W. Rüdiger, Thea Koske-Westphal, W. Wöhler & E. Passarge

  2. I. Medizinische Klinik, Universität Hamburg, Hamburg, Germany

    W. Byarugaba, H. W. Rüdiger, Thea Koske-Westphal, W. Wöhler & E. Passarge

Authors
  1. W. Byarugaba
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  2. H. W. Rüdiger
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  3. Thea Koske-Westphal
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  4. W. Wöhler
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  5. E. Passarge
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Additional information

This investigation was supported in part by grants from the Deutsche Forschungsgemeinschaft. W. B. is recipient of a scholarship from Deutscher Akademischer Austauschdienst.

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Byarugaba, W., Rüdiger, H.W., Koske-Westphal, T. et al. Toxicity of antibiotics on cultured human skin fibroblasts. Hum Genet 28, 263–267 (1975). https://doi.org/10.1007/BF00278556

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  • DOI: https://doi.org/10.1007/BF00278556

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