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Genetical analysis of rifampicin resistant mutants of E. coli K12

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Summary

E. coli rifampicin-resistant (rif-r) mutants were divided into two conventional groups: A, resistant to 50–100μg/ml of rifampicin both on complete and minimal medium and B, sensitive to these concentrations of rifampicin on minimal, but resistant on complete medium. RNA polymerase from rif-r-A mutants is resistant to high concentrations of rifampicin in vitro while the enzyme from rif-r-B mutants but slightly if at all differs from the wild-type enzyme in its response to the drug.

All rif-r-A and rif-r-B mutants are closely linked and map between argH and thi on E. coli K 12 chromosome. We failed to isolate any rifampicin-resistant mutants which would map outside this region.

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Authors and Affiliations

  1. Kurchatov Institute of Atomic Energy, Moscow, USSR

    S. Z. Mindlin, T. S. Ilyina, T. A. Voeykova & V. V. Velkov

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  1. S. Z. Mindlin
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  2. T. S. Ilyina
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  3. T. A. Voeykova
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  4. V. V. Velkov
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Communicated by D. Goldfarb

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Mindlin, S.Z., Ilyina, T.S., Voeykova, T.A. et al. Genetical analysis of rifampicin resistant mutants of E. coli K12. Molec. Gen. Genet. 115, 115–121 (1972). https://doi.org/10.1007/BF00277290

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  • DOI: https://doi.org/10.1007/BF00277290

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