Summary
The apolipoprotein gene cluster on human chromosome 19 (APOC1, APOC2, APOE) has been localised by pulsed-field gel electrophoresis to within 200 kb of a chronic lymphocytic leukemia-associated translocation breakpoint. A restriction map covering 1300 kb around these loci has been constructed and contains two polymorphic MluI sites, which appear to show Mendelian inheritance. The orientation of the map on the chromosome has been established as 19cen CLL breakpoint-APOC2-19qter. Pedigree analysis using APOC2, a probe derived from the CLL breakpoint, and other localised markers on 19q suggests that the myotonic dystrophy locus is distal to APOC2 on 19q.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brook JD, Skinner M, Roberts SH, Rettig WJ, Almond JW, Shaw DJ (1988) Further mapping of markers around the centromere of human chromosome 19. Genomics 1:320–328
Chu G, Vollarth D, Davis RW (1986) Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234:1582–1585
Harley HG, Brook JD, Jackson CL, Glaser T, Walsh KV, Sarfarazi M, Kent R, Lager M, Koch M, Harper PS, Levenson R, Housman DE, Shaw DJ (1988) Localisation of a human Na+, K+ AT-Pase alpha subunit to chromosome 19q12-q13.2 and linkage to the myotonic dystrophy locus. Genomics 3:380–384
Harper PS (1979) Myotonic dystrophy. Saunders, Philadelphia
Hulsebos T, Wieringa B, Hochstenbach R, Smeets D, Schepens J, Oerlemans F, Zimmer J, Ropers HH (1986) Toward early diagnosis of myotonic dystrophy: construction and characterisation of a somatic cell hybrid with a single human der(19) chromosome. Cytogenet Cell Genet 43:47–56
Korneluk RG, MacLeod HL, McKeithan TW, Brook JD, McKenzie AE (1989) A chromosome 19 clone from a translocation breakpoint shows tight linkage and linkage disequilibrium with myotonic dystrophy. Genomics 4:146–151
Lindsay S, Bird AP (1987) Use of restriction enzymes to detect potential gene sequences in mammalian DNA. Nature 327:336–338
McKeithan TW, Rowley JD, Shows TB, Diaz MO (1987) Cloning of the chromosome translocation breakpoint of the t(14,19) in chronic lymphocytic leukemia. Proc Natl Acad Sci USA 84:9257–9260
Myklebost O, Rogne S (1988) A physical map of the apolipoprotein gene cluster on human chromosome 19. Hum Genet 78:244–247
Nakamura Y, Lathrop M, O'Connell P, Leppert M, Lalouel JM, White R (1988) A primary map of ten DNA markers and two serological markers for human chromosome 19. Genomics 3:67–71
Schonk D, Coerwinkel-Driessen M, Dalen I van, Oerlemans F, Smeets B, Schepens J, Hulsebos T, Cockburn D, Boyd Y, Davis M, Rettig W, Shaw D, Roses A, Ropers H, Wieringa B (1989) Definition of subchromosomal intervals around the myotonic dystrophy gene region at 19q. Genomics 4:384–396
Scott J, Knott TJ, Shaw DJ, Brook JD (1985) Localisation of genes encoding apolipoproteins CI, CII and E to the p13-cen region of human chromosome 19. Hum Genet 71:144–146
Sharpe CR, Sidoli A, Shelley CS, Lucero MA, Shoulders CC, Baralle FE (1984) Human apolipoproteins AI, AII, CII and CIII: cDNA sequences and mRNA abundance. Nucleic Acids Res 12:3917–3932
Shaw DJ, Eiberg H (1987) Report of the commitee for chromosomes 17, 18 and 19. (9th International Workshop on Human Gene Mapping). Cytogenet Cell Genet 46:242–256
Shaw DJ, Meredith AL, Sarfarazi M, Huson SM, Brook JD, Myklebost O, Harper PS (1985) The apolipoprotein CII gene: subchromosomal localisation and linkage to the myotonic dystrophy locus. Hum Genet 70:271–273
Shaw DJ, Meredith AL, Sarfarazi M, Harley HG, Huson SM, Brook JD, Bufton L, Litt M, Mohandas T, Harper PS (1986) Regional localisations and linkage relationships of seven RFLPs and myotonic dystrophy on chromosome 19. Hum Genet 74:262–266
Stallings RL, Olson E, Strauss AW, Thompson LH, Bachinski LL, Siciliano MJ (1988) Human creatine kinase genes on chromosomes 15 and 19, and proximity of the gene for the muscle form to the genes for apolipoprotein C2 and excision repair. Am J Hum Genet 43:144–151
Van Ommen GJB, Verkerk JMH (1986) Restriction analysis of chromosomal DNA in a size range up to two million base pairs by pulsed field gradient electrophoresis. In: Davies KE (ed) Human genetic diseases — a practical approach. IRL Press, Oxford, pp 113–133
Vollrath D, Davis RW (1987) Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields. Nucleic Acids Res 15:7865–7876
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shaw, D.J., Harley, H.G., Brook, J.D. et al. Long-range restriction map of a region of human chromosome 19 containing the apolipoprotein genes, a CLL-associated translocation breakpoint, and two polymorphic MluI sites. Hum Genet 83, 71–74 (1989). https://doi.org/10.1007/BF00274152
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00274152