Summary
Four Japanese wheat varieties, three crossable and one non-crossable with Hordeum bulbosum, were pollinated with maize pollen of 5 genotypes. By the application of 2,4-dichlorophenoxyacetic acid after pollination, embryos kept developing on wheat plants until 14 days after pollination. The frequency of embryo formation was significantly different among the maize genotypes, varying from 18.0% to 31.9%, but not among the wheat varieties. By bagging spikes with flag leaves the frequency of embryo formation was increased by about 7%. Ten- to twelve-day-old embryos gave higher frequencies of plant formation (83.6%) than 14-day-old embryos(50.0%). All 6 regenerated plants investigated cytologically were found to be haploid. Twelve of the 14 colchicine-treated plants produced florets setting seeds. The overall efficiency of our procedure is considered to be higher than that reported by Laurie and Bennett (1988).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Folk DE, Kasha KJ (1981) Comparison of the crossability of rye(Secale cereale) and Hordeum bulbosum onto Wheat (Triticum aestivum). Can J Genet Cytol 23:81–88
Inagaki M (1985) Chromosome doubling of the wheat haploids obtained from crosses with Hordeum bulbosum L. Japan J Breed 35:193–195
Inagaki M (1986) Crossability of Japanese wheat cultivars with Hordeum bulbosum L. Japan J Breed 36:363–370
Inagaki M, Henry Y, De Buyser J (1987) Comparison of haploid production efficiency through anther culture and intergeneric crossing in three wheat varieties and their F1 hybrids. Japan J Breed 37:474–478
Laurie DA, Bennett MD (1986) Wheat x Maize hybridization. Can J Genet Cytol 28:313–316
Laurie DA, Bennett MD (1987) The effect of the crossability loci Kr1 and Kr2 on fertilization frequency in hexaploid wheat x maize crosses. Theor Appl Genet 73:403–409
Laurie DA, Bennett MD (1988) The production of haploid wheat plants from wheat x maize crosses. Theor Appl Genet 76:393–397
Marshall DR, Molnàr-Làng M, Ellison FW (1983) Effects of 2,4-D on parthenocarpy and cross-compatibility in wheat. Cereal Res Comm 11:213–219
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nishibayashi S, Kaeriyama J (1986) Structural stability of chromosomes in Rice (Oryza sativa L.) plants regenerated from somatic tissue culture. Plant Tissue Culture Lett 3:31–34
Pickering RA (1982) The effect of pollination bag type on seed quality and size in Hordeum inter- and intraspecific hybridization. Euphytica 31:439–449
Sitch LA, Snape JW (1986) The influence of the Hordeum bulbosum and the wheat genotype on haploid production in wheat (Triticum aestivum). Z Pflanzenzüchtg 96:304–319
Author information
Authors and Affiliations
Additional information
Communicated by C. T. Harms
Rights and permissions
About this article
Cite this article
Suenaga, K., Nakajima, K. Efficient production of haploid wheat (Triticum aestivum) through crosses between Japanese wheat and maize (Zea mays). Plant Cell Reports 8, 263–266 (1989). https://doi.org/10.1007/BF00274125
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00274125