Summary
Gamete production in the hybridogenetic species hybrid Rana esculenta (Rana ridibunda X Rana lessonae) is preceded by a premeiotic elimination of the R. lessonae genome and subsequent duplication of the remaining R. ridibunda genome, so that only ridibunda chromosomes enter a quasi normal meiosis, and only ridibunda gametes are formed. This is demonstrated by differences in genome specific centromere fluorescence and electrophoretic patterns between somatic and gonadal tissue.
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Literature
Berger, L. (1968): Morphology of the F1 generation of various crosses within Rana esculenta complex. Acta Zool. Kracow 13, 301–324
Cimino, M.C. (1972a): Egg production, polyploidization, and evolution in a diploid all-female fish of the genus Poeciliopsis. Evolution 26, 294–306
Cimino, M.C. (1972b): Meiosis in triploid all-female fish (Poeciliopsis, Poeciliidae). Science 175, 1484–1486
Comings, D.E. (1975): Mechanism of chromosome banding VIII. Hoechst 33 258-DNA interaction. Chromosoma 52, 229–243
Cuellar, O. (1976): Cytology of meiosis in the triploid gynogenetic salamander Ambystoma tremblayi. Chromosoma 58, 355–364
Fawcett, D.W. (1972): Observations on cell differentiation and organelle continuity in spermatogenesis. In: Proc. Int. Symp: The Genetics of the Spermatozoon (eds. Beatty, R.A.; Gluecksohn-Waelsch, S.), pp. 37–68. Edinburgh, New York: Plenum Press
Galgano, M.; Morescalchi, A. (1966): Differenze sessuali nel diplotene di Rana esculenta L. Rend. Acad. Sci. fis. mat. Soc. Naz. Sci. Lett. Arti 33, 79–87
Graf, J.D.; Karch, F.; Moreillon, M.-C. (1977): Biochemical variation in the Rana esculenta complex: A new hybrid form related to Rana perezi and Rana ridibunda. Experientia 33, 1582–1584
Graf, J.-D.; Müller, W.P. (1979): Experimental gynogenesis provides evidence of hybridogenetic reproduction in the Rana esculenta complex. Experientia 35, 1574–1577
Greilhuber, J. (1977): Why plant chromosomes do not show G-bands. Theor. Appl. Genet. 50, 121–124
Günther, R. (1975): Untersuchungen der Meiose bei Männchen von Rana ridibunda Pall., Rana lessonae Cam. und der Bastardform „Rana esculenta” L. (Anura). Biol. Zbl. 94, 277–294
Heppich, S. (1978): Hybridogenesis in Rana esculenta: C-band karyotypes of Rana ridibunda, Rana lessonae and Rana esculenta. Z. zool. Syst. Evol.-Forsch. 16, 27–39
Heppich, S. (1980): Chromosomenbänder bei Wasserfröschen: Untersuchungen zum Hybridcharakter und zur hybridoge-netischen Fortpflanzung von Rana esculenta (Amphibia, Anura). Diss. Univers. Wien
Heppich, S.; Tunner, H.G. (1979): Chromosomal constitution and C-banding in homotypic Rana esculenta crosses. Mitt. Zool. Mus. Berlin 55, 111–114
John B.; Miklos, G.L.G. (1979): Functional aspects of satellite DNA and heterochromatin. Int. Rev. Cytol. 58, 1–114
Latt, S.A.; Wohlleb, J.C. (1975): Optical studies of the interaction of 33 258 Hoechst with DNA, chromatin and metaphase chromosomes. Chromosoma 52, 297–316
Macgregor, H.C.; Uzzell, T. (1964): Gynogenesis in salamanders related to Ambystoma jeffersonianum. Science 135, 1043–1045
Morescalchi, A.; Galgano, M. (1973): Meiotic chromosomes and their taxonomic value in Amphibia Anura. Caldasia 11, 41–50
Phillips, D.M. (1974): Spermiogenesis. New York, London: Acad. Press
Schmid, M. (1978): Chromosome banding in Amphibia II. Constitutive heterochromatin and nucleolus organizer regions in Ranidae, Microhylidae and Rhacophoridae. Chromosoma 68, 131–148
Schweizer, D. (1976): Dapi fluorescence of plant chromosomes prestained with actinomycin D. Exp. Cell Res. 102, 408–413
Tunner, H.G. (1973): Das Albumin und andere Bluteiweiße bei Rana ridibunda Pallas, Rana lessonae Camerano, Rana esculenta Linné und deren Hybriden. Z. zool. Syst. Evol.- Forsch. 11, 219–233
Tunner, H.G. (1974): Die klonale Struktur einer Wasserfrosch-population. Z. zool. Syst. Evol.-Forsch. 12, 309–314
Tunner, H.G. (1979): The inheritance of morphology and electrophoretic markers from homotypic crosses of the hybrido-genetic Rana esculenta. Mitt. Zool. Mus. Berlin 55, 89–109
Tunner, H.G. (1980): Kreuzungsexperimente mit Wasserfröschen aus österreichischen und polnischen Mischpopulationen (Rana lessonae + Rana esculenta). Eine Analyse biochemischer und morphologischer Merkmale. Z. zool. Syst. Evol.-Forsch. 18, 257–297
Tunner, H.G.; Dobrowsky, M.-Th. (1976): Zur morphologischen, serologischen und enzymologischen Differenzierung von Rana lessonae und der hybridogenetischen Rana esculenta aus dem Neusiedlersee (Österreich, Burgenland). Zool. Anz. 197, 6–22
Tunner, H.G.; Heppich, S. (1981): Premeiotic genome exclusion during oogenesis in the common edible frog Rana esculenta. Naturwissenschaften 68, 207–208
Uzzell, T.; Berger, L. (1975): Electrophoretic phenotypes of Rana ridibunda, Rana lessonae, and their hybridogenetic associate, Rana esculenta. Proc. Acad. Nat. Sci., Philadelphia 127, 13–24
Walker, P.M.B. (1971): Origin of satellite DNA. Nature 219, 306–308
Wickbom, T. (1945): Cytological studies on Dipnoi, Urodela, Anura and Emys. Hereditas 35, 241–346
Yamamoto, M.; Miklos, G.L.G. (1978): Genetic studies on heterochromatin in Drosophila melanogaster and their implications for the functions of satellite DNA. Chromosoma 66, 71–98
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Communicated by F. Mechelke
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Heppich, S., Tunner, H.G. & Greilhuber, J. Premeiotic chromosome doubling after genome elimination during spermatogenesis of the species hybrid Rana esculenta . Theoret. Appl. Genetics 61, 101–104 (1982). https://doi.org/10.1007/BF00273874
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DOI: https://doi.org/10.1007/BF00273874