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Ribosomal proteins

V. Comparison of protein patterns of 70S and 80S ribosomes from various plants by polyacrylamide gel electrophoresis

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Summary

A comparison of the protein patterns of the 70S and 80S ribosomes from various plants, E. coli and yeast by disc-gel electrophoresis has shown the following relations: 1. There is a greater similarity between chloroplast ribosomes from various plants than between chloroplast and cytoplasmic ribosomes obtained from the same plant. 2. The protein patterns of the cytoplasmic ribosomes from bean, spinach and tobacco are more similar to each other than when compared to that of wheat germ. 3. At least one band is common to cytoplasmic ribosomes from all plants tested. 4. Only very few bands with identical mobilities are observed between chloroplast and E. coli ribosomes and between cytoplasmic plant and yeast ribosomes.

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References

  • Anderson, L. A., and R. M. Smillie: Binding of chloramphenicol by ribosomes from chloroplasts. Biochem. biophys. Res. Commun. 23, 535 (1966).

    Google Scholar 

  • Boardman, N. K., R. I. B. Francki, and S. G. Wildman: Protein synthesis by cell-free extracts of tobacco leaves. III. Comparison of the physical properties and protein synthesizing activities of 70S chloroplast and 80S cytoplasmic ribosomes. J. molec. Biol. 17, 470 (1966).

    Google Scholar 

  • Bruskov, V. I., and M. S. Odintsova: Comparative electron microscopic studies of chloroplast and cytoplasmic ribosomes. J. molec. Biol. 32, 471 (1968).

    Google Scholar 

  • Chersi, A., M. Dzionara, D. Donner, and H. G. Wittmann: Ribosomal Proteins. IV. Isolation, amino acid compositions, peptide maps and molecular weights of yeast ribosomal proteins. Molec. Gen. Genetics 101, 82 (1968).

    Google Scholar 

  • Clark, M. F., R. E. F. Matthews, and R. K. Ralph: Ribosomes and polyribosomes in Brassica pekinensis. Biochim. biophys. Acta (Amst.) 91, 289 (1964).

    Google Scholar 

  • Kaltschmidt, E., M. Dzionara, D. Donner, and H. G. Wittmann: Ribosomal proteins. I. Isolation, amino acid compositions, molecular weights and peptide mapping of proteins from E. coli ribosomes. Molec. Gen. Genetics 100, 364 (1967).

    Google Scholar 

  • Leboy, P. S., E. C. Cox, and J. G. Flaks: The chromosomal site specifying a ribosomal protein in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 52, 1367 (1964).

    Google Scholar 

  • Lyttleton, J. W.: Isolation of ribosomes from spinach chloroplasts. Exp. Cell Res. 26, 312 (1962).

    Google Scholar 

  • —: Protein constituents of plant ribosomes. Biochim. biophys. Acta (Amst.) 154, 145 (1968).

    Google Scholar 

  • Stutz, E., and H. Noll: Characterization of cytoplasmic and chloroplast polysomes in plants: Evidence for three classes of ribosomal RNA in nature. Proc. nat. Acad. Sci. (Wash.) 57, 774 (1967).

    Google Scholar 

  • Wolfe, F. H., and C. M. Kay: Physicochemical, chemical, and biological studies on wheat embryo ribosomes. Biochemistry 6, 2853 (1967).

    Google Scholar 

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Communicated by G. Melchers

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Janda, HG., Wittmann, H.G. Ribosomal proteins. Molec. Gen. Genet. 103, 238–243 (1968). https://doi.org/10.1007/BF00273693

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  • DOI: https://doi.org/10.1007/BF00273693

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