Summary
A comparison of the protein patterns of the 70S and 80S ribosomes from various plants, E. coli and yeast by disc-gel electrophoresis has shown the following relations: 1. There is a greater similarity between chloroplast ribosomes from various plants than between chloroplast and cytoplasmic ribosomes obtained from the same plant. 2. The protein patterns of the cytoplasmic ribosomes from bean, spinach and tobacco are more similar to each other than when compared to that of wheat germ. 3. At least one band is common to cytoplasmic ribosomes from all plants tested. 4. Only very few bands with identical mobilities are observed between chloroplast and E. coli ribosomes and between cytoplasmic plant and yeast ribosomes.
Similar content being viewed by others
References
Anderson, L. A., and R. M. Smillie: Binding of chloramphenicol by ribosomes from chloroplasts. Biochem. biophys. Res. Commun. 23, 535 (1966).
Boardman, N. K., R. I. B. Francki, and S. G. Wildman: Protein synthesis by cell-free extracts of tobacco leaves. III. Comparison of the physical properties and protein synthesizing activities of 70S chloroplast and 80S cytoplasmic ribosomes. J. molec. Biol. 17, 470 (1966).
Bruskov, V. I., and M. S. Odintsova: Comparative electron microscopic studies of chloroplast and cytoplasmic ribosomes. J. molec. Biol. 32, 471 (1968).
Chersi, A., M. Dzionara, D. Donner, and H. G. Wittmann: Ribosomal Proteins. IV. Isolation, amino acid compositions, peptide maps and molecular weights of yeast ribosomal proteins. Molec. Gen. Genetics 101, 82 (1968).
Clark, M. F., R. E. F. Matthews, and R. K. Ralph: Ribosomes and polyribosomes in Brassica pekinensis. Biochim. biophys. Acta (Amst.) 91, 289 (1964).
Kaltschmidt, E., M. Dzionara, D. Donner, and H. G. Wittmann: Ribosomal proteins. I. Isolation, amino acid compositions, molecular weights and peptide mapping of proteins from E. coli ribosomes. Molec. Gen. Genetics 100, 364 (1967).
Leboy, P. S., E. C. Cox, and J. G. Flaks: The chromosomal site specifying a ribosomal protein in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 52, 1367 (1964).
Lyttleton, J. W.: Isolation of ribosomes from spinach chloroplasts. Exp. Cell Res. 26, 312 (1962).
—: Protein constituents of plant ribosomes. Biochim. biophys. Acta (Amst.) 154, 145 (1968).
Stutz, E., and H. Noll: Characterization of cytoplasmic and chloroplast polysomes in plants: Evidence for three classes of ribosomal RNA in nature. Proc. nat. Acad. Sci. (Wash.) 57, 774 (1967).
Wolfe, F. H., and C. M. Kay: Physicochemical, chemical, and biological studies on wheat embryo ribosomes. Biochemistry 6, 2853 (1967).
Author information
Authors and Affiliations
Additional information
Communicated by G. Melchers
Rights and permissions
About this article
Cite this article
Janda, HG., Wittmann, H.G. Ribosomal proteins. Molec. Gen. Genet. 103, 238–243 (1968). https://doi.org/10.1007/BF00273693
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00273693