Summary
Following both chemical and electrical fusion of protoplasts of a dihaploid line of potato (Solanum tuberosum), (PDH40), with those of the wild species, Solanum brevidens, 11 and 40 somatic hybrid plants, respectively were obtained. Fifteen of these somatic hybrid genotypes and the two parents were studied further in a small field trial to assess field performance and phenotypic variability. In the UK, somatic hybrid plants are classified as genetically engineered organisms, and the UK Advisory Committee on Genetic Manipulation have imposed various restrictions on field experiments. Examination of the somatic hybrids in the field showed extensive phenotypic variability, and no two genotypes were identical. Some of the variation reflected changes in chromosome numbers, but other factors were also involved. Half the somatic hybrid genotypes produced tubers in the field, although the tubers were smaller and differed morphologically from those of PDH40. The results of the study suggest that the extent of somaclonal variation manifested in somatic hybrids is greater than that found in protoplast-derived plants of potato. The implications of this observation and the current regulations concerning field experiments of somatic hybrid plants in the UK are discussed.
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References
ACGM/HSE/Note 3 (1986) Advisory Committee on Genetic Manipulation. The planned release of genetically manipulated organisms for agricultural and environmental purposes. Guidelines for risk assessment and for the notification of proposals for such work. Health and Safety Executive, London/UK
Austin S, Baer MA, Helgeson JP (1985a) Transfer of resistance to potato leaf roll virus from Solarium brevidens into Solanum tuberosum by somatic fusion. Plant Sci 39:75–82
Austin S, Baer M, Ehlenfeldt M, Kasmierczak PJ, Helgeson JP (1985b) Intra-specific fusions in Solanum tuberosum. Theor Appl Genet 71:172–175
Austin S, Ehlenfeldt MK, Baer MA, Helgeson JP (1986) Somatic hybrids produced by protoplast fusion between S. tuberosum and 5. brevidens: phenotypic variation under field conditions. Theor Appl Genet 71:682–690
Evans DA, Sharp WR (1986) Applications of somaclonal variation. Biotechnology 4:528–533
Evans DA, Bravo JA, Kut SA and Flick CE (1983) Genetic behaviour of somatic hybrids in the genus Nicotiana: N. otophora and N. tabacum and N. sylvestris and N. tabacum. Theor Appl Genet 65:93–101
Evans DA, Sharp WR, Mediana-Filho HPC (1984) Somaclonal and gametoclonal variation. Am J Bot 71:759–774
Evans NE, Foulger D, Farrer L, Bright SWJ (1986) Somaclonal variation in explant-derived potato clones over three tuber generations. Euphytica 35:353–361
Fish N (1988) Somatic hybridisation of potato (Solanum tuberosum L.). Ph.D Thesis, University of London
Fish N, Karp A (1986) Improvements in regeneration from protoplasts of potato and studies on chromosome stability. 1. The effect of initial culture media. Theor Appl Genet 72:405–412
Fish N, Karp A, Jones MGK (1987) Improved isolation of dihaploid Solanum tuberosum protoplasts and the production of somatic hybrids between dihaploid 5. tuberosum and S. brevidens. In Vitro 23:575–580
Fish N, Karp A, Jones MGK (1988) Production of somatic hybrids by electrofusion in Solanum. Theor Appl Genet 76:260–266
Gavazzi G, Tonelli G, Todesco G, Arreghini E, Raffaldi F, Vecchio F, Barbuzzi G, Biasini MG, Sala F (1987) Somaclonal variation versus chemically induced mutagenesis in tomato (Lycopersicon esculentum L.) Theor Appl Genet 74:733–738
Gibson RW, Jones MGK, Fish N (1988) Resistance to potato leaf roll virus and potato virus Y in somatic hybrids between dihaploid S. tuberosum and S. brevidens. Theor Appl Genet 76:113–117
Hauptli H, Newell N, Goodman RM (1985) Genetically engineered plants: environmental issues. Biotechnology 3:437–442
Huffman F, Adachi T (1981) “Arabidobrassica”: chromosomal recombination and morphogenesis in asymmetric inter- generic hybrid cells. Planta 153:586–593
Jones MGK (1987) Use of protoplast fusion and somaclonal variation in potato breeding. In: Jellis GJ, Richardson DE (eds) The production of new potato varieties. Cambridge University Press, London, pp 315–326
Karp A, Bright SWJ (1985) On the causes and origins of somaclonal variation. In: Miflin BJ (ed) Oxford surveys of plant molecular and cell biology, vol. 2. Cambridge University Press, London, pp 199–234
Kemble RJ, Barsby TL, Wong RSC, Shepard JF (1986) Mitochondrial DNA rearrangements in somatic hybrids of Solanum tuberosum and Solanum brevidens. Theor Appl Genet 72:787–793
Landsmann J, Uhrig H (1985) Somaclonal variation in Solanum tuberosum detected at the molecular level. Theor Appl Genet 71:500–505
Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214
Maine MJ de (1978) Field resistance to late blight and potato root eelworm in group tuberosum dihaploids. Euphytica 27:305–315
Maine MJ de (1982) An evaluation of the use of dihaploids and unreduced gametes in breeding for quantitative resistance to potato pathogens. J Agric Sci 99:79–83
Matern U, Strobel G, Shepard J (1978) Reaction to phytotoxins in a potato population derived from mesophyll protoplasts. Proc Natl Acad Sci USA 75:4935- 4939
National Institute of Agricultural Botany (1975) Guide to the identification of potato varieties. Cambridge University Press, London
Pfeiffer TW, Bingham ET (1984) Comparisons of alfalfa somaclonal and sexual derivatives from the same genetic source. Theor Appl Genet 67:263–266
Powell W, Caligari PDS, Dunwell JM (1986) Field performance of lines derived from haploid and diploid tissues of Hordeum vulgare. Theor Appl Genet 72:458–465
Quemada H, Roth EJ, Lark KG (1987) Changes in methylation of tissue cultured soybean cells detected by digestion with the restriction enzymes Hpall and Mspl. Plant Cell Rep 6:63–66
Ross H (1986) Potato breeding — problems and perspectives. Parey, Berlin Hamburg
Secor GA, Shepard JF (1981) Variability of protoplast-derived potato clones. Crop Sci 21:102–105
Shepard JF, Bidney D, Shahin E (1980) Potato protoplasts in crop improvement. Science 208:17–24
Thomas E, Bright SWJ, Franklin J, Lancaster VA, Miflin BJ, Gibson RJ (1982) Variation amongst protoplast-derived potato plants (Solanum tuberosum cv. ‘Maris Bard’) Theor Appl Genet 62:65–68
Uijtewaal BA, Posthumus E, Suurs LCJM, Jacobsen E, Hermsen JGTh (1987) Relative performance of monohaploid potato clones and their diploid parents at plant level and after protoplast isolation and subsequent fusion. Theor Appl Genet 74:451–458
Wenzel G, Schieder O, Przewozny T, Sopory SK, Melchers G (1979) Comparison of single cell culture derived Solanum tuberosum L. plants and a model for their application in breeding programs. Theor Appl Genet 55:49–55
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Communicated by Y. Y. Gleba
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Fish, N., Steele, S.H. & Jones, M.G.K. Field assessment of dihaploid Solatium tuberosum and S. brevidens somatic hybrids. Theoret. Appl. Genetics 76, 880–886 (1988). https://doi.org/10.1007/BF00273676
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DOI: https://doi.org/10.1007/BF00273676