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Glyphosate tolerant flax plants from Agrobacterium mediated gene transfer

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Abstract

Agrobacterium tumefaciens carrying a disarmed Ti-plasmid vector containing a chimeric NPT-II gene and a glyphosate resistance plant-derived 5-enolpyruvylshikimate-3-phosphate synthase gene was used to transform flax hypocotyl tissues. Transformed shoots could be regenerated from the inoculated tissue and were proven to be transgenic by the combination of leaf callus assays, nopaline assays and progeny tests. Co-segregation was observed in the progeny for kanamycin and glyphosate resistance.

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Authors and Affiliations

  1. Crop Development Centre, University of Saskatchewan, S7N 0W0, Saskatoon, Canada

    Mark C. Jordan & Alan McHughen

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  1. Mark C. Jordan
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  2. Alan McHughen
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Communicated by A. R. Gould

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Jordan, M.C., McHughen, A. Glyphosate tolerant flax plants from Agrobacterium mediated gene transfer. Plant Cell Reports 7, 281–284 (1988). https://doi.org/10.1007/BF00272543

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  • DOI: https://doi.org/10.1007/BF00272543

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