Summary
Fragile sites on murine chromosomes were induced by the antimetabolites methotrexate (MTX), fluorodeoxyuridine (FdU), and aphidicolin (APD). To facilitate chromosome identification the analysis was performed on chromosomes of a CD/CD mouse that possesses nine pairs of Robertsonian translocation chromosomes of known arm composition. The pattern of induced fragile sites was rather similar for the different antimetabolites used. Many of them, e.g., 2B, 3B, 5B and 9D, are included in Giemsa-negative bands. On the X chromosome a fragile site was mapped to the region XC/D. Comparative mapping data with human fragile sites have been informative in most instances. Conservation of synteny within known linkage groups seems very likely.
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References
Avner P, Amar L, Arnaud D, Hanauer A, Cambrou J (1987) Detailed ordering on markers localising to the Xq26-Xqter region of human X chromosome by the use of an interspecific Mus spretus mouse cross. Proc Natl Acad Sci USA (in press)
Barbi G, Steinbach P, Vogel W (1984) Nonrandom distribution of methotrexate-induced aberrations on human chromosomes. Detection of further folic acid sensitive fragile sites. Hum Genet 68: 290–294
Berger R, Bloomfield CD, Sutherland GR (1985) Report of the committee on chromosome rearrangements in neoplasia and on fragile sites, 8th International Workshop on Human Gene Mapping (1985). Cytogenet Cell Genet 40: 490–535
Buckle VJ, Edwards JH, Evans EP, Jonasson JA, Lyon MF, Peters J, Searle AG, Wedd NS (1984) Chromosome maps of man and mouse II. Clin Genet 26: 1–11
Capanna E, Gropp A, Winking H, Noak G, Civitelli MV (1976) Robertsonian metacentrics in the mouse. Chromosoma 58: 341–353
Davisson MT, Roderick TH (1986) Linkage map of the mouse. Mouse News Lett 75: 6–7
Djalali M, Barbi G, Steinbach P (1985) Folic acid sensitive fragile sites are not limited to the human karyotype. Demonstration of nonrandom gaps and breaks in the Persian vole Ellobius lutescens Th. inducible by methotrexate, fluorodeoxyuridine, and aphidicolin. Hum Genet 70: 183–185
Dutrillaux B (1979) Chromosomal evolution of primates: tentative phylogeny from Microcebus murinus (prosimian) to man. Hum Genet 48: 251–314
Dutrillaux B, Couturier J, Viegas Pegnignont E (1981) Chromosomal evolution in primates. Chromosomes Today 7: 176–191
Glover TW, Berger C, Coyle J, Echo B (1984) DNA polymerase alpha inhibition by aphidicolin induces gaps and breaks at common fragile sites in human chromosomes. Hum Genet 67: 136–142
Goldman MA, Holmquist GP, Gray MC, Caston A, Nag A (1984) Replication timing of genes and middle repetitive sequences. Science 224: 686–692
Hecht F, Sutherland GR (1984) Fragile sites and cancer breakpoints. Cancer Genet Cytogenet 12: 179–181
von Kiel K, Hameister H, Somssich IE, Adolph S (1985) Early replication banding reveals a strongly conserved functional pattern in mammalian chromosomes. Chromosoma 93: 69–76
Lalley PA, McKusick VA (1985) Report of the committee on comparative mapping. 8th International Workshop on Human Gene Mapping 1985. Cytogenet Cell Genet 40: 536–566
Ledbetter DH, Airhart SD, Nussbaum RL (1986) Caffeine enhances fragile (X) expression in somatic cell hybrids. Am J Med Genet 23: 445–455
Lin MS, Takabayashi T, Wilson MG, Marchese CA (1984) An in vitro and in vivo study of a BrdU-sensitive fragile site in the Chinese hamster. Cytogenet Cell Genet 38: 211–215
Nadeau JH, Taylor BA (1984) Length of chromosomal segments conserved since divergence of man and mouse. Proc Natl Acad Sci USA 81: 814–818
Natarajan AT, Raposa T (1975) Heterochromatin and chromosome aberrations. A comparative study of three mouse cell lines with different karyotype and heterochromatin distribution. Hereditas 80: 83–90
Nussbaum RS, Ledbetter DH (1986) Fragile X syndrome: a unique mutation in man. Ann Rev Genet 20: 109–145
O'Brien J, Nash WG (1982) Genetic mapping in mammals: chromosome map of domestic cat. Science 216: 257–265
Opitz J, Sutherland GR (1984) Conference report. International Workshop on the fragile X and X-linked mental retardation. Am J Med Genet 17: 5–94
Pearson PL, Roderick TH (1982) Report of the committee on comparative mapping. Human Gene Mapping 6. Cytogenet Cell Genet 32: 208–220
Sanz MM, Jenkins EC, Brown T, Davisson MT, Kevin MJ, Roderick TH, Silverman WP, Wisniewski HM (1986) Mouse chromosome fragility. Am J Med Genet 23: 491–509
Schmid M, Ott G, Haaf T, Scheres JMJC (1985) Evolutionary conservation of fragile sites induced by 5-azacytidine and 5-azadeoxycytidine in man, gorilla, and chimpanzee. Hum Genet 71: 342–350
Stallings RL, Munk AC, Longmire JL, Jett JH, Wilder ME, Siciliano MJ, Adair GM, Crawford BD (1985) Oncogenes and linkage groups: conservation during mammalian chromosome evolution. Chromosoma 92: 156–163
Yunis JJ, Kuo MT, Saunders GF (1977) Localization of sequences specifying messenger RNA to light stained G-bands of human chromosomes. Chromosoma 61: 335–344
Yunis JJ, Soreng AL (1984) Constitutive fragile sites and cancer. Science 226: 1199–1204
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Djalali, M., Adolph, S., Steinbach, P. et al. A comparative mapping study of fragile sites in the human and murine genomes. Hum Genet 77, 157–162 (1987). https://doi.org/10.1007/BF00272384
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DOI: https://doi.org/10.1007/BF00272384