Summary
Differential staining of sister chromatids in BrdU-substituted human chromosomes is demonstrated by an ammoniacal silver carbonate procedure. With this method the chromosomes exhibit a subchromatid structure. Because proteolytic treatment indicated that the silver carbonate binds the chromosome proteins, changes of these components may be inferred in the BrdU-substituted chromosomes. Sister chromatid exchanges could be identified.
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References
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This study was supported in part by research grant 12/119/77 from the Instituto Nacional de Previsión (Seguridad Social).
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Goyanes, V.J. Differential silver carbonate staining of sister chromatids in BrdU-substituted chromosomes. Hum Genet 40, 205–208 (1978). https://doi.org/10.1007/BF00272302
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DOI: https://doi.org/10.1007/BF00272302