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Immunocytochemical determination of ploidy class-dependent bromodeoxyuridine incorporation in rat liver parenchymal cells after partial hepatectomy

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Summary

Immunocytochemistry of bromodeoxyuridine (BrdU) incorporated in DNA was performed on cryostat sections of rat liver and on isolated hepatocytes after partial hepatectomy using a two-step labeling technique. The method enabled the detection of S-phase nuclei in both tissue preparations. Quantification of the number of labeled nuclei in sections showed that the number of nuclei in S-phase increased from 0.3% in control liver to about 36% at 24 h after partial hepatectomy. The detection of BrdU in isolated hepatocytes showed the same labeling index of binuclear diploid, mononuclear tetraploid and binuclear tetraploid cells. A special role for mononuclear diploid cells in proliferation did not seem to occur.

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Authors and Affiliations

  1. Laboratory of Cell Biology and Histology, University of Amsterdam, Academic Medical Centre, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands

    W. M. Frederiks, F. Marx, C. J. F. van Noorden & J. James

  2. Department of Experimental Internal Medicine, University of Amsterdam, Academic Medical Centre, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands

    R. A. F. M. Chamuleau

Authors
  1. W. M. Frederiks
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  2. F. Marx
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  3. R. A. F. M. Chamuleau
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  4. C. J. F. van Noorden
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  5. J. James
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Frederiks, W.M., Marx, F., Chamuleau, R.A.F.M. et al. Immunocytochemical determination of ploidy class-dependent bromodeoxyuridine incorporation in rat liver parenchymal cells after partial hepatectomy. Histochemistry 93, 627–630 (1990). https://doi.org/10.1007/BF00272205

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  • DOI: https://doi.org/10.1007/BF00272205

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