Summary
A unified biochemical and genetic study has led to the location of the gene for erythromycin resistance in 4 mutants of Chalamydomonas reinhardi and the identification of the chloroplast ribosomal protein altered in these strains. Tetrad analyses of crosses involving the Mendelian mutants ery-M1a, ery-M1b, ery-M1c, and ery-M1d indicate that they are closely-linked alleles which map to nuclear linkage group XI. The proteins of the 52s subunits of wild-type and the 4 ery-M1 mutants were compared by one-and two-dimensional gel electrophoresis. Protein LC6 from wild-type differs in net charge at pH 5 from the homologous component found in each of the mutants. The LC6 in ery-M1b is also 30% smaller in molecular weight than the wild-type protein. The variety of altered forms of LC6 observed and the allelic nature of the genetic determinants strongly support the view that the nuclear locus for erythromycin resistance of the ery-M1 group is the structural gene for the chloroplast ribosomal protein LC6.
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Communicated by H. G. Wittmann
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Davidson, J.N., Hanson, M.R. & Bogorad, L. An altered chloroplast ribosomal protein in ery-M1 mutants of Chlamydomonas reinhardi. Molec. Gen. Genet. 132, 119–129 (1974). https://doi.org/10.1007/BF00272177
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DOI: https://doi.org/10.1007/BF00272177