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Gene conversion spectrum of 15 mutants giving post-meiotic segregation in the b2 locus of Ascobolus immersus

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Summary

The conversion spectrum of fifteen mutants giving post-meiotic segregations and located in the b2 locus of Ascobolus immersus was studied in 77 mutant x wild-type crosses. These mutants all yield aberrant 4:4 asci, mutants located in the right portion of the locus yielding more aberrant 4:4 than left mutants. The basic frequency of conversion is higher in the left portion. The frequency of hybrid DNA, its symmetrical or asymmetrical distribution and the frequency of correction of the mismatch in hybrid DNA were estimated. The left region shows a higher frequency of hybrid DNA formation than the right region. The fraction of hybrid DNA with a symmetrical distribution tends to increase from left to right in the locus. The frequencies of mismatch correction show considerable variation from one mutant to another and have no relationship to their location. The implications of these observations on the molecular models of genetic recombination are discussed.

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Author notes

  1. N. Paquette

    Present address: Department de Biologie, Université Laval, Cité universitaire, Québec 10°, Canada

Authors and Affiliations

  1. Laboratoire de Génétique, bât. 400, Université Paris-Sud, F-91405, Orsay-Cedex, France

    N. Paquette & J.-L. Rossignol

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  1. N. Paquette
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  2. J.-L. Rossignol
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Communicated by W. Gajewski

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Paquette, N., Rossignol, JL. Gene conversion spectrum of 15 mutants giving post-meiotic segregation in the b2 locus of Ascobolus immersus . Molec. Gen. Genet. 163, 313–326 (1978). https://doi.org/10.1007/BF00271961

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  • DOI: https://doi.org/10.1007/BF00271961

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