Summary
The TOL plasmid pWWO gives rise to the Tol− plasmid pWWO-8 due to a specific excision event involving 40 kb of TOL DNA. This event occurs by means of reciprocal recombination involving a pair of directly repeated 1.4 kb sequences. This 40 kb segment is not involved as such in the formation in vivo of hybrid plasmids between TOL and resistance plasmids, notably RP4.
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Communicated by E. Bautz
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Meulien, P., Downing, R.G. & Broda, P. Excision of the 40kb segment of the TOL plasmid from Pseudomonas putida mt-2 involves direct repeats. Molec. Gen. Genet. 184, 97–101 (1981). https://doi.org/10.1007/BF00271202
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DOI: https://doi.org/10.1007/BF00271202