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Regional replication of the bacterial chromosome induced by derepression of prophage lambda

III. Role of the replication in escape synthesis of gal operon

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Summary

Recent evidence suggests that the escape synthesis of gal operon following derepression of the prophage λ in Escherichia coli K 12 involves transcription originating at the λ promoter (PL) to extend through gal under the conditions in which λ DNA replication is prevented. Whether the observed expression of gal is due to transcription initiating at PL or at the bacterial promoter for gal (Pgal) was examined in the case of λ DNA replication being normal. The experiments are based on that two types of transcription are distinguished from each other by the following properties: 1. Pgal-promoted transcription is inhibited by chloramphenicol, while PL-promoted transcription is not. 2. PL-promoted transcription suppresses the polar effect caused by nonsense mutation in a bacterial gene, while Pgal-promoted transcription does not do so. The results have suggested that gal escape synthesis in λ-induced lysogen results from transcription which initiates not only at PL but also at Pgal. The Pgal-promoted transcription may be a consequence, direct or indirect, of the concomitant replication of gal DNA.

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Authors and Affiliations

  1. Research Unit for Molecular Genetics, Keio University School of Medicine, Shinjuku, Tokyo, Japan

    Toshio Fukasawa & Kazuko Obonai

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  1. Toshio Fukasawa
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  2. Kazuko Obonai
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Communicated by T. Yura

The second article of this series is in Molec. gen. Genet. 147, 71–78 (1976)

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Fukasawa, T., Obonai, K. Regional replication of the bacterial chromosome induced by derepression of prophage lambda. Molec. Gen. Genet. 159, 185–190 (1978). https://doi.org/10.1007/BF00270892

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  • DOI: https://doi.org/10.1007/BF00270892

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