Skip to main content
Log in

Selection of the most potent Bacillus sphaericus strains based on activity ratios determined on three mosquito species

  • Applied Microbiology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

The larvicidal power of more than 180 Bacillus sphaericus strains belonging to six H serotypes has been assayed on Culex pipiens, Anopheles stephensi and Aedes aegypti under standardized conditions. The most potent strains are distributed into serotype H5a5b, generally toxic to the three mosquito species, and serotypes H6 and H25, toxic to C. pipiens and A. stephensi. Strains of serotypes 26a26b and H2a2b are much less toxic and most often only on C. pipiens. The relative potency of each strain can be expressed by specific titres on the different mosquito species and by activity ratios derived from such titres.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barjac H de (1989) Classification of B. sphaericus strains and comparative toxicity to mosquito larvae. In: Barjac H de, Sutherland D (eds) Bacterial control of mosquitoes and blackflies, chapter 14, Rutgers University Press

  • Barjac H de, Charles J-F (1983) Une nouvelle toxine active sur les moustiques, présente dans des inclusions cristallines produites par Bacillus sphaericus. CR Acad Sci [III]:905–910

    Google Scholar 

  • Barjac H de, Larget-Thiery I (1984) Characteristics of IPS82 as standard for biological assay of Bacillus thuringiensis H14 preparations. WHO Mimeogr Doc WHO/VBC/84.892

  • Barjac H de, Larget-Thiery I, Cosmao Dumanoir V, Ripouteau H (1985) Serological classification of Bacillus sphaericus strains in relation with toxicity to mosquito larvae. Appl Microbiol Biotechnol 21:85–90

    Google Scholar 

  • Barjac H de, Thiery I, Cosmao Dumanoir V, Frachon E, Laurent Ph, Ofori J, Charles J-F, Hamon S (1988) Another Bacillus sphaericus serotype harbouring strains very toxic to mosquito larvae: serotype H6. Ann Inst Pasteur Microbiol 139:363–377

    Google Scholar 

  • Baumann P, Unterman BM, Bauman L, Broadwell AH, Abbene SJ, Bowditch RD (1985) Purification of the larvicidal toxin of Bacillus sphaericus and evidence for high-molecular-weight precursors. J Bacteriol 163:738–747

    Google Scholar 

  • Baumann L, Broadwell AH and Baumann P (1988) Sequence analysis of the Mosquitocidal Toxin Genes Encoding 51.4 and 41.9 Kilodalton Proteins from Bacillus sphaericus 2362 and 2297. J Bacteriol 170:2045–2050

    Google Scholar 

  • Davidson EW (1982) Purification and properties of soluble cytoplasmic toxin from the mosquito pathogen Bacillus sphaericus strain 1593. J Invertebr Pathol 39:6–9

    Google Scholar 

  • Dulmage HD (1981) Insecticidal activity of isolates of Bacillus thuringiensis and their potential for pest control. In: Burges D (ed) Microbial contro of pests and plant diseases 1970–1980, Academic Press, London, pp 193–222

    Google Scholar 

  • Kalfon A, Larget-Thiery I, Charles J-F, Barjac H de (1983) Growth, sporulation and larvicidal activity of Bacillus sphaericus. Eur J Appl Microbiol Biotechnol 18:168–173

    Google Scholar 

  • Kellen WR, Clark TB, Lindegren JE, Ho BC, Rogoff MH, Singer S (1965) Bacillus sphaericus Neide as a pathogen of mosquitoes. J Invertebr Pathol 7:442–448

    Google Scholar 

  • Lysenko O, Davidson EW, Lacey LA, Yousten AA (1985) Five new mosquito larvicidal strains of Bacillus sphaericus from non-mosquito origins. J Am Mosq Control Assoc 1:369–371

    Google Scholar 

  • Myers PS, Yousten AA (1980) Localization of the mosquitolarval toxin of Bacillus sphaericus 1593. Microbiology 39:1205–1211

    Google Scholar 

  • Singer S (1977) Isolation and development of bacterial pathogens of vectors. In: Biological regulation of vectors. HEW Washington, D.C. Publication no. (NIH) 77-1180, pp 3–18

  • Thiery I (1988) Evaluation et contrôle au laboratoire du pouvoir entomopathogène de Bacillus thuringiensis var. israelensis et de Bacillus sphaericus sur larves de Culicidae (Diptères, Nématocères). Thèse de Docteur en Science de la Vie de l'Université Paris XI

  • Weiser J (1984) A mosquito-virulent Bacillus sphaericus in adult Simulium damnosum from Northern Nigeria. Zentralbl Mikrobiol 139:57–60

    Google Scholar 

  • Wickremesinghe RSB, Mendis C (1980) Bacillus sphaericus spore from Sri Lanka demonstrating rapid larvicidal activity on Culex quinquefasciatus. Mosq News 40:387–389

    Google Scholar 

  • Yousten AA, Davidson EW (1982) Ultrastructural analysis of spores and parasporal crystals formed by Bacillus sphaericus. Appl Environ Microbiol 44:1449–1455

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thiery, I., de Barjac, H. Selection of the most potent Bacillus sphaericus strains based on activity ratios determined on three mosquito species. Appl Microbiol Biotechnol 31, 577–581 (1989). https://doi.org/10.1007/BF00270798

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00270798

Keywords

Navigation