Skip to main content
SpringerLink
Log in

Studies on the polyphosphate cycle in Candida utilis

Effect of dilution rate and nitrogen source in continuous culture

  • Applied Microbiology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

Candida utilis cells were grown in continuous culture in a medium with ammonium or arginine as the nitrogen source. Arginine produced a marked change in the amount of polyphosphates and arginine in whole cells and vacuoles, as well as in the ratio of the concentrations of these substances. The specific growth rate (μ) which in continuous culture is equivalent to the dilution rate (D), affects the amount and chain length of the polyphosphates and also the arginine content of the vacuoles and whole cells. Thus, if D is increased the amount of polyphosphates per milligram protein is increased. There is apparently a direct and linear relationship between D, the specific growth rate (μ) and the polyphosphate content. Changes in D also affect the length of the polyphosphate chain, and the relationship is inverse. At low growth rates, two types of chain were observed, one of approximately 35 phosphate units and the other of 5 units. At high growth rates phosphorus was not stored as longchain polyphosphates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Cowie DB, McClure FT (1959) Metabolic pools and the synthesis of macromolecules. Biochim Biophys Acta 31:236–245

    Google Scholar 

  • Drews G (1962) The cytochemistry of polyphosphates. Colloq Int Cent Natl Rech Sci 106:533–539

    Google Scholar 

  • Dürr M, Urech K, Boller Th, Wiemken A, Schwencke J, Nagy M (1979) Sequestration of arginine by polyphosphates in vacuoles of yeast (S. cerevisiae). Arch Microbiol 121:169–175

    Google Scholar 

  • Harold FM (1966) Inorganic polyphosphate in biology: structure, metabolism and function. Bacteriol Rev 30:772–785

    Google Scholar 

  • Huber-Wälchli V, Wiemken A (1979) Differential extraction of soluble pools from the cytosol and the vacuoles of yeast (Candida utilis) using DEAE-dextran. Arch Microbiol 120:141–149

    Google Scholar 

  • Indge KJ (1968a) Metabolic lysis of yeast protoplasts. J Gen Microbiol 51:433–440

    Google Scholar 

  • Indge KJ (1968b) Polyphosphate of the yeast cell vacuole. J Gen Microbiol 51:447–455

    Google Scholar 

  • Langen P, Liss E, Lohmann K (1962) Art, Bildung und Umsatz der Polyphosphate der Hefe. Colloq Int Cent Natl Rech Sci 106:603–612

    Google Scholar 

  • Leloir LF, Cardini CE (1957) Characterization of phosphorous compounds by acid lability. In: Colowick SP, Kaplan NO (eds) Methods in Enzymology, vol 3. Academic Press, New York, pp 840–850

    Google Scholar 

  • Lindberg O, Ernster L (1955) Determination of inorganic phosphorous compounds by phosphate analysis. Methods Biochem Anal 3:1–22

    Google Scholar 

  • Liss E, Langen P (1962) Versuche zur Polyphosphat-Überkompensation in Hefezellen nach Polyphosphatverarmung. Arch Microbiol 41:382–392

    Google Scholar 

  • Lohmann K (1931) Darstellung der Adenylpyrophorsäure aus Muskulatur. Biochem Z 233:460–469

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • Ohnishi ST, Gall RS (1978) Characterization of the catalyzed phosphate assay. Anal Biochem 88:347–356

    Google Scholar 

  • Peterson GL (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83:346–356

    Google Scholar 

  • Ramos F, Thuriaux P, Wiame JM, Bechet J (1970) The participation of ornithine and citrulline in the regulation of arginine metabolism in Saccharomyces cerevisiae. Eur J Biochem 12:40–47

    Google Scholar 

  • Stebbin N (1971) Growth changes in pool and macromolecular components of Schizosaccharomyces pombe during the cell cycle. J Cell Sci 9:701–717

    Google Scholar 

  • Urech K, Dürr M, Boller Th, Wiemken A (1978) Localization of polyphosphate in vacuoles of Saccharomyces cerevisiae. Arch Microbiol 116:275–278

    Google Scholar 

  • Wiemken A, Dürr M (1974) Characterization of amino acids pools in the vacuolar compartment of Saccharomyces cerevisiae. Arch Microbiol 101:45–57

    Google Scholar 

  • Wiemken A, Nurse P (1973) Isolation and characterization of the amino acids pools located within the cytoplasm and vacuoles of Candida utilis. Planta 109:293–306

    Google Scholar 

  • Wilden W van der, Matile Ph, Schellenberg M, Meyer J, Wiemken A (1973) Vacuolar membranes: isolation from yeast cells. Z Naturforsch 28c:416–421

    Google Scholar 

  • Zalokar M (1961) Kinetics of amino acid uptake and protein synthesis in Neurospora. Biochim Biophys Acta 46:423–432

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Planta Piloto de Procesos Industriales Microbiológicos-PROIMI, Av. Belgrano y Pje. Caseros, 4000, Tucumán, Argentina

    Carlos Germán Núñez & Danley Arturo Saturnino Callieri

Authors
  1. Carlos Germán Núñez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Danley Arturo Saturnino Callieri
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Núñez, C.G., Saturnino Callieri, D.A. Studies on the polyphosphate cycle in Candida utilis . Appl Microbiol Biotechnol 31, 562–566 (1989). https://doi.org/10.1007/BF00270795

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00270795

Keywords

Search

Navigation