Summary
Bacteria able to secrete proteins efficiently into the growth medium occur relatively rarely amongst Gram-negative species. However, the increasing technological interest in protein secretion has focused attention on this process. We have demonstrated that Myxococcus xanthus actively secretes protein. The number of proteins secreted is quite large, but the total amount is strictly regulated and remains constant under conditions that change the specific activities of some of the secreted enzymes. Tn5-insertion mutants were obtained which were impaired in what seems to be the control system for protein secretion. Two of the mutants displayed increased levels of extracellular protein.
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References
Bowden G, Mothibeli MA, Robb FT, Wodds DR (1982) Regulation of Hut enzymes and extracellular protease activities in Vibrio alginolyticus hut mutants. J Gen Microbiol 128:2041–2045
Campos JM, Geisselsoder J, Zusman D (1978) Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus. J Mol Biol 119:167–178
Coleman G (1981) Plciotropic compensation in the regulation of extracellular protein formation by low αtoxin producing variant of Staphyloccocus aureus (WOOD 46). J Gen Microbiol 122:11–15
Dworkin M (1962) Nutritional requirements for vegetative growth of Myxococcus xanthus. J Bacteriol 84:250–257
Dworkin M (1972) The myxobacteria: New directions in studies of procaryotic development. CRC Crit Rev Microbiol 1:435–452
Glenn AR (1976) Production of extracellular proteins by bacteria. Annu Rev Microbiol 80:41–62
Gnospelius G (1978) Purification and properties of an extracellular protease from Myxococcus virescens. J Bacteriol 133:17–25
Hart BA, Zahler SA (1966) Lytic enzyme produced by Myxococcus xanthus. J Bacteriol 92:1632–1637
Haska G, Stahl S (1971) Variants of Myxococcus virescens exhibiting dispersed growth. Growth and production of extracellular enzymes and slime. Physiol Plant 24:136–142
Horney DL, Webster DA (1974) Deoxyribonuclease: a sensitive assay using radial diffusion in agarose containing methyl green-DNA complex. Biochem Biophys Acta 247:54–71
Howard S, Buckley J (1983) Intracellular accumulation of extracellular proteins by pleiotropic export mutants of Aeromonas hydrophila. J Bacteriol 154:413–418
Irschik H, Gerth K, Kremmer T, Steinmetz H, Reichenbach M (1983) The myxovalagins, new peptide antibiotics from Myxococcus xanthus. I Cultivation, isolation and some chemical and biological properties. J Antibiotics 36:6–12
Jensen SE, Fecycz IT, Stemke GW, Campbell JN (1980) Demonstration of a cell-associated, inactive precursor of an exocellular protease produced by Pseudomonas aeruginosa. Can J Microbiol 26:87–93
Kaiser D, Manoil C, Dworkin M (1979) Myxobacteria: cell interactions genetics and development. Ann Rev Microbiol 33:595–639
Kuner JM, Avery L, Berg DE, Kaiser D (1981) Use of transposon Tn5 in the genetic analysis of Myxococcus xanthus. In: Schlessinger D (ed) Microbiology. A S M Washington D C, p 128
Kuner JM, Kaiser D (1981) Introduction of transposon Tn5 into Myxococcus xanthus for analysis of developmental and other nonselectable mutants. Proc Natl Acad Sci USA 78:425–429
Kuner JM, Kaiser D (1982) Fruiting body morphogenesis in submerged cultures of Myxococcus xanthus. J Bacteriol 151:458–461
Lazzaroni JC, Portalier RC (1979) Isolation and preliminary characterisation of periplasmic leaky mutants of Escherichia coli K12. FEMS Microbiol Lett 5:411–416
Lazzaroni JC, Portalier RC (1981) Genetic and biochemical characterisation of periplasmic leaky mutants of Escherichia coli K12. J Bacteriol 145:1351–1358
Millet J (1970) Characterisation of proteinases secreted by Bacillus subtilis marburg strain during sporulation. J Appl Bacteriol 33:207–219
Nicaud JM (1983) Etude de mutants affectés dans l'excretion d'enzymes extracellulaires chez Myxococcus xanthus. Thèse Université de Technologie de Compiègne
Nielsen JBK, Mezes PSF, Lampen JO (1983) Secretion by Pseudomonas aeruginosa: fate of a cloned gram-positive lipoprotein deletion mutant. J Bacteriol 156:559–566
Rosenberg E, Keller K, Dworkin M (1977) Cell density dependent growth of Myxococcus xanthus on casein J Bacteriol 129:770–777
O'Connor K, Zusman DR (1983) Coliphage P1, mediated transduction of cloned DNA from Escherichia coli to Myxococcus xanthus: Use for complementation and recombination analysis. J Bacteriol 155:317–329
Sedmak J, Grossberg S (1977) A rapid, sensitive and versatile assay for protein using coomassie brilliant blue G250. Anal Biochem 79:544–552
Shimkets LJ, Gill RE, Kaiser D (1983) Development cell interactions in Myxococcus xanthus and the SpoC Locus. Proc Natl Acad Sci USA 80:1406–1410
Springer W, Goebel W (1980) Synthesis and secretion of haemolysin by Escherichia coli. J Bacteriol 144:53–59
Sudo S, Dworkin M (1972) Bacteriolytic enzymes produced by Myxococcus xanthus. J Bacteriol 110:236–245
Torriani A (1960) Influence of inorganic phosphate in the formation of phosphatase by Escherichia coli Biochem Biophys Acta 38:460–479
Van Tiel-Menkveld GJ, Rezee A, De Graff FK (1979) Production and secretion of cloacin DF 13 by Escherichia coli harbouring plasmid Clo DF13. J Bacteriol 140:415–423
Watson BF, Dworkin M (1968) Comparative intermediary metabolism of vegetative cells and microcysts of Myxococcus xanthus. J Bacteriol 96:1465–1473
Wretlind B, Sjoberg L, Wadstrom T (1977) Proteases deficient mutants of Pseudomonas aeruginosa: pleiotropic changes in activity of other extracellular enzymes. J Gen Microbiol 103:329–336
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Nicaud, J.M., Breton, A., Younes, G. et al. Mutants of Myxococcus xanthus impaired in protein secretion: an approach to study of a secretory mechanism. Appl Microbiol Biotechnol 20, 344–350 (1984). https://doi.org/10.1007/BF00270598
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DOI: https://doi.org/10.1007/BF00270598