Summary
Isoelectric focusing (pH 4.0–5.0) of serum α2HS-glycoprotein on polyacrylamide gels has been found to be a useful tool in population genetics and forensic science. Using this method, we isolated three common types, α2HS 1-1, α2HS 2-1 and α2HS 2-2, and showed that α2HS types are determined by two autosomal codominant alleles, α2 HS 1 and α2 HS 2. The method is simple, fast and easy to perform. Results of typing for the two alleles, α2 HS 1 and α2 HS 2, are described for a Japanese population sample (n=1003).
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Anderson NL, Anderson NG (1977) High resolution two-dimensional electrophoresis of human plasma proteins. Proc Natl Acad Sci USA 74:5421–5425
Anderson NL, Anderson NG (1979) Microheterogeneity of serum transferrin, haptoglobin and α2HS glycoprotein examined by high resolution two-dimensional electrophoresis. Biochem Biophys Res Commun 88:258–265
Cox DW, Andrews BJ (1983) Silver stain immunofixation for α2HS glycoprotein: A new method for detection of protein heterogeneity. In: Stathakos D (ed) Electrophoresis ′82. Walter de Gruyter, Berlin, pp 243–247
Heremans JF (1960) Les globulines sériques du système gamma. Arscia, Brussels, p 103
von Oss CJ, Gillman CF, Bronson PM, Border JR (1974) Opsonic properties of human serum alpha-2 HS glycoprotein. Immunol Commun 3:329–335
Schmid K, Bürgi W (1961) Preparation and properties of the human plasma Ba-α2-glycoproteins. Biochim Biophys Acta 47:440–453
Schultze HE, Heide K, Haupt H (1962) Charakterisierung eines niedermolekularen α2-Mukoids aus Humanserum. Naturwissenschaften 49:15
Umetsu K, Kashimura S, Ikeda N, Suzuki T (1983) Classification of α2HS-glycoprotein (α2HS) types by isoelectric focusing. Z Rechtsmed 91:33–35
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Umetsu, K., Kashimura, S., Ikeda, N. et al. A new α2HS-glycoprotein typing by isoelectric focusing. Hum Genet 67, 70–71 (1984). https://doi.org/10.1007/BF00270561
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DOI: https://doi.org/10.1007/BF00270561