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Uncharged tRNA inhibits guanosine 3′,5′-bis (diphosphate) 3′-pyrophosphohydrolase [ppGppase], the spoT gene product, from Escherichia coli

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Summary

The spoT gene product from Escherichia coli, the guanosine 3′,5′-bis(diphosphate) 3′-pyrophosphohydrolase [ppGppase] catalyzes the specific release of pyrophosphate from the 3′-position of guanosine 3′,5′-bis(diphosphate) [ppGpp]; this reaction is significantly inhibited in the presence of uncharged tRNA Pheyeast . Little or no inhibition is observed with Phe-tRNAPhe, tRNAPhe-CpCpAoxi-red or ribosomal RNA (16S and 23S).

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References

  • Chinali, G., Lion, R., Ofengand, J.: Role of the aminoacyl end of transfer RNA in the allosteric control of guanosine pentaphosphate synthesis by the stringent factor-ribosome complex of Escherichia coli. Biochemistry 17, 2761–2768 (1978)

    Google Scholar 

  • Cramer, F., Haar, F. von der, Schlimme, E.: Über das Aminoacy-lierungsverhalten chemisch modifizierter Phenylalanin-spezifischer Transfer-Ribonucleinsäure aus Hefe: (1) Glykolspaltung und Reduktion zum Diol an der 3′terminalen Ribose. FEBS Lett. 2, 136–139 (1968)

    Google Scholar 

  • Raué, H., Cashel, M.: Regulation of RNA synthesis in Escherichia coli. III. Degradation of guanosien 5′-diphosphate 3′-diphosphate in cold-shocked cells. Biochem. Biophys. Acta 383, 290–304 (1975)

    Google Scholar 

  • Richter, D.: Synthesis and degradation of the pleiotropic effector guanosine 3′,-5′Bis(diphosphate) in bacteria. In: Regulation of macromolecular synthesis by low molecular weight mediators (Koch, G., and Richter, D. eds.), pp. 85–94, New York: Academic Press 1979a

    Google Scholar 

  • Richter, D.: In vitro synthesis and decay of guanosine 3′,5′bis(diphosphate) [ppGpp]. In: Ribosomes (Chambliss, G., Craven, G.R., Davies, J., Davis, K., Kahan, L., Nomura, M., eds.), pp. 743–765. Baltimore: University Park Press 1979b

    Google Scholar 

  • Richter, D., Fehr, S., Harder, R.: The guanosine 3′,5′-bis(diphosphate) (ppGpp) cycle. Comparison of synthesis and degradation of guanosine 3′,5′-bis(diphosphate) in various bacterial systems. Eur. J. Biochem. 99, 57–64 (1979)

    Google Scholar 

  • Sprinzl, M., Kucharzewski, M., Hobbs, J.B., Cramer, F.: Specificity of elongation factor Tu from Escherichia coli with respect to attachment of the amino acid to the 2′ or 3′-hydroxy group of the terminal adenosine of tRNA. Eur. J. Biochem. 78, 55–61 (1977)

    Google Scholar 

  • Sprinzl, M., Richter, D.: Free 3′-OH group of the terminal adenosine of the tRNA molecule is essential for the synthesis in vitro of guanosine tetraphosphate and pentaphosphate in a ribosomal system from Escherichia coli. Eur. J. Biochem. 71, 171–176 (1976)

    Google Scholar 

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Authors and Affiliations

  1. Institut für Physiologische Chemie, Abteilung Zellbiochemie, Universität Hamburg, Martinistr. 52, D-2000, Hamburg 20, Federal Republic of Germany

    Dietmar Richter

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  1. Dietmar Richter
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Communicated by K. Isono

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Richter, D. Uncharged tRNA inhibits guanosine 3′,5′-bis (diphosphate) 3′-pyrophosphohydrolase [ppGppase], the spoT gene product, from Escherichia coli . Molec. Gen. Genet. 178, 325–327 (1980). https://doi.org/10.1007/BF00270479

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  • DOI: https://doi.org/10.1007/BF00270479

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