Abstract
Fern gametophytes initiated from spores grew successfully when immobilised in reticulate polyurethane foam in liquid shake cultures. Colonisation of this matrix was complete seven days after medium inoculation and appeared to be a completely passive process. Yield (dry weight) was greater than that from liquid grown cultures.
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Brouers M, Hall DO (1987) In: Webb C, Mavituna F (eds) Plant and animal cells, process possibilities, Ellis Harwood Ltd, Chichester, England pp 193–202
Debergh PC (1983) Physiol Plant 59: 270–276
Dyer AF (1979) In: Dyer AF (ed) The Experimental Biology of Ferns, Academic Press, London, pp 253–305
Dyer AF, King MAL (1979) In: Dyer AF (ed) The Experimental Biology of Ferns, Academic Press, London, pp 307–354
Gemmrich AR (1986) J Plant Physiol 125: 157–166
Hickok LG, Warne LK, Slocum MK (1987) Am J Bot 74: 1304–1316
Howland GP (1972) Physl Plant 26: 264–270
Ishida BK (1988) Plant Cell Reports 7: 270–273
Kordan HA (1988) Biochem Physiol Pflanz 183: 355–359
Lindsey K, Yeoman MM (1984) Planta 162: 495–501
Lindsey K, Yeoman MM, Black GM, Mavituna F (1983) FEBS Lett 15: 143–149
Mavituna F, Park JM (1985) Biotechnol Lett 7: 637–640
Miller JH (1968) Botan Rev 34: 361–440
Moore GT (1903) J Applied Micr 6: 2309–2314
Näf U (1979) In: Dyer AF (ed) The Experimental Biology of Ferns, Academic Press, London, pp 436–470
Mullem A, Bruce D, Hall DO (1983) Biotechnol Lett 5: 365–368
Partenen CR (1972) Bot Gaz 133: 287–292
Schraudolf H (1986) In: Bopp M, Knoop B, Rademacher W (eds) Proceedings 12th International Conference on Plant Growth Substances, Springer, Berlin, pp 270–274
Smith DL, Robinson PM (1971) New Phytol 70: 1043–1052
Yeoman MM (1988) Ann Bot 60: 157–174
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Communicated by I. Potrykus
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Douglas, G.E., Sheffield, E. A new technique for the culture of fern gametophytes. Plant Cell Reports 8, 632–634 (1990). https://doi.org/10.1007/BF00270071
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DOI: https://doi.org/10.1007/BF00270071