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Mutants in yeast affecting ethidium bromide induced ρ −formation and their effects on transmission and recombination of mitochondrial genes

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Summary

A series of mutants called ebi, less inducible by ethidium bromide than the parental strain for the ρ+→ρ− mutation have been isolated after E.M.S. mutagenesis. Some of the ebi mutants also show an important accumulation of ρ− cells, in the absence of ethidium bromide. Ebi mutations are nuclearly inherited as shown by meiotic segregation. The effects of these mutants on the transmission and recombination of mitochondrial genes among the diploid progeny of crosses have been studied. Some of the ebi mutants show a non coordinated transmission of the oli1 mitochondrial marker with respect to other mitochondrial markers unexpected for homosexual crosses. This bias which is independent from ω will be discussed in relation to the segregation and recombination. No significant decrease of the frequency of recombinants has been detected.

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Abbreviations

E.B.:

Ethidium bromide

E.M.S.:

Ethyl méthane sulfonate

CS/CR :

Allelic forms of the rib 1 locus conferring chloramphenicol sensitivity/resistance

ES/ER :

Allelic forms of the rib 3 locus conferring erythromycine sensitivity/resistance

OR/OR :

Allelic forms of the oli 1 locus conferring oligomycin sensitivity/resistance

PS/PR :

Allelic forms of the par 1 locus conferring paromomycine sensitivity/resistance

DS/DR :

Allelic forms of the diu 1 or diu 2 loci conferring diuron sensitivity/resistance

CS/CR :

Allelic forms of the mitochondrial ω locus

ρ+ :

“grande” or respiratory competent cells

ρ− :

“petite” or cytoplasmic respiratory deficient cells

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  1. Centre de Génétique Moléculaire du C.N.R.S., F-91190, Gif-sur-Yvette, France

    Geneviève Dujardin & Bernard Dujon

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  1. Geneviève Dujardin
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  2. Bernard Dujon
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Communicated by W. Gajewski

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Dujardin, G., Dujon, B. Mutants in yeast affecting ethidium bromide induced ρ −formation and their effects on transmission and recombination of mitochondrial genes. Molec. Gen. Genet. 171, 205–214 (1979). https://doi.org/10.1007/BF00270006

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  • DOI: https://doi.org/10.1007/BF00270006

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