Abstract
Somatic embryos of carrot, Daucus carota L. ‘Royal Chantenay’, were chilled at 4°C for the last 3 days of development in order to harden torpedo stage embryos to increase embryo survival during desiccation. ABA levels in chilled and non-chilled embryos were measured using a polyclonal radioimmunoassay and a monoclonal enzyme-linked immunosorbent assay (ELISA). The monoclonal ELISA is the preferred technique due to superior sensitivity and specificity. ABA levels, measured by either technique, were similar in chilled and non-chilled embryos. The relative water content was lower in chilled embryos than in non-chilled embryos and chilling altered protein secretion of one cell line.
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Abbreviations
- ABA:
-
abscisic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BHT:
-
2,5-di-tert-butyl-4-methyl phenol
- BSA:
-
bovine serum albumin
- ELISA:
-
enzyme-linked immunosorbent assay.
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Communicated by I. K. Vasil
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Spencer, T.M., Kitto, S.L. Measurement of endogenous ABA levels in chilled somatic embryos of carrot by immunoassay. Plant Cell Reports 7, 352–355 (1988). https://doi.org/10.1007/BF00269936
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DOI: https://doi.org/10.1007/BF00269936