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Plant regeneration from shoot callus of rosewood (Dalbergia latifolia Roxb.)

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Abstract

In vitro propagation of trees using cell, tissue and organ culture is a fast emerging area. We report here the clonal propagation of Indian rosewood (Dalbergia latifolia Roxb.) from shoot callus cultures of 5 year old trees. Bud regeneration was obtained on MS media supplemented with BA and NAA. About 35% of the cultures showed organogenesis. Shoots measuring about 3–5 cm can be excised and rooted in White's medium supplemented with 1–2 mg/L IAA. Rooted plants were successfully established in soil.

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Abbreviations

BA:

Benzyladenine

CM:

Coconut milk

2,4-D:

2,4 dichlorophenoxyacetic acid

IAA:

Indoleacetic acid

IBA:

Indolebutyric acid

K:

Kinetin

NAA:

Naphthaleneacetic acid

PVP-360:

Polyvinyl pyrrolidone

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Authors and Affiliations

  1. Microbiology and Cell Biology Laboratory, Indian Institute of Science, 560 012, Bangalore, India

    G. Lakshmi Sita & S. Chattopadhyay

  2. Botany Department, Bangalore University Jnanabharathi, 560 056, Bangalore, India

    D. H. Tejavathi

Authors
  1. G. Lakshmi Sita
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  2. S. Chattopadhyay
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  3. D. H. Tejavathi
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Additional information

Communicated by O.L. Gamborg

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Lakshmi Sita, G., Chattopadhyay, S. & Tejavathi, D.H. Plant regeneration from shoot callus of rosewood (Dalbergia latifolia Roxb.). Plant Cell Reports 5, 266–268 (1986). https://doi.org/10.1007/BF00269818

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  • DOI: https://doi.org/10.1007/BF00269818

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