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Suspension and protoplast culture of hexaploid wheat (Triticum aestivum L.)

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Abstract

Suspension cultures have been initiated from embryogenic callus of hexaploid wheat (Triticum aestivum L.). Most commonly, these “suspensions” are composed of callus-like clusters (up to 2 mm in diameter). Two rapidly-growing lines (MBE6 and C82d) have been obtained, which consist of smaller aggregates of cytoplasmic cells, and these have been maintained for more than 4 years. These lines show very limited morphogenetic capacity and only a single plantlet has been regenerated, from line MBE6, after 9 months in culture. Protoplasts isolated from line MBE6 are unable to divide, but protoplasts from line C82d consistently undergo sustained divisions to form callus or secondary cell suspensions.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

Murashige and Skoog (1962) medium

References

  • Chu C, Wang S, Sun S, Hsu C, Yin K, Chu C, Bi F (1975) Sci Sinica 18:659–668

    Google Scholar 

  • Dale PJ (1983) Experientia Suppl 46:31–41

    Google Scholar 

  • Fujimura T, Sakurai M, Akagi H, Negishi T, Hirose A (1985) Plant Tissue Culture Letters 2:74–7

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50:151–158

    Google Scholar 

  • Jones MGK (1985) In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture, Nijhoff/Junk, Dordrecht, pp 204–230

    Google Scholar 

  • Kao KN, Michyaluk MR (1975) Planta 126:105–110

    Google Scholar 

  • King PJ (1980) Int Rev Cytol, Supp 11A:25–53

    Google Scholar 

  • King PJ, Potrykus I, Thomas E (1978) Physiol Veg 16:381–399

    Google Scholar 

  • Larkin PJ (1976) Planta 128:213–216

    Google Scholar 

  • Lu C, Vasil V, Vasil IK (1981) Z Pflanzenphysiol 104:311–318

    Google Scholar 

  • Maddock SE, Lancaster VA, Risiott R, Franklin J (1983) J Exp Bot 34:915–926

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    Google Scholar 

  • Potrykus I, Harms CT, Lorz H, Thomas E (1977) Mol Gen Genet 156:347–350

    Google Scholar 

  • Schenk RV, Hildebrandt AC (1972) Can J Bot 50:199–204

    Google Scholar 

  • Shillito RD, Paszkowski J, Potrykus I (1983) Plant Cell Reports 2:244–247

    Google Scholar 

  • Srinivasan C, Vasil IK (1986) J Plant Physiol In press

  • Toriyama K, Hinata K (1985) Plant Sci 41:179–183

    Google Scholar 

  • Vasil V, Vasil IK (1980) Theor Appl Genet 56:97–99

    Google Scholar 

  • Vasil V, Wang DY, Vasil IK (1983) Z Pflanzenphysiol 111:233–239

    Google Scholar 

  • Yamada Y, Zhi-Q Y, Ding-Tai T (1986) Plant Cell Reports 5:85–88

    Google Scholar 

Download references

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Authors and Affiliations

  1. Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts, UK

    S. E. Maddock

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  1. S. E. Maddock
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Communicated by I.K. Vasil

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Maddock, S.E. Suspension and protoplast culture of hexaploid wheat (Triticum aestivum L.). Plant Cell Reports 6, 23–26 (1987). https://doi.org/10.1007/BF00269731

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  • DOI: https://doi.org/10.1007/BF00269731

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