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In vitro cultures of Cinchona species

Precursor feeding of C. ledgeriana root organ suspension cultures with l-Tryptophan

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Abstract

The uptake of l-[methylene-14C]-tryptophan from culture medium into root organs of Cinchona ledgeriana and the subsequent incorporation of the radiolabel into quinine and quinidine is reported. In addition, feeding unlabelled l-tryptophan at levels of 500mg/l to the cultures results in a 5-fold increase in the yields of both quinoline alkaloids.

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References

  • Anderson LA, Keene AT and Phillipson JD (1982a) Planta Med. 46: 25–27

    Google Scholar 

  • Anderson LA, Keene AT and Phillipson JD (1982b) J Pharm Pharmac 34: 45P

  • Bisset NG (1982) Proceedings of The Fourth Meeting of Turkish Pharmacognosists, Eskisehir, pp53–65

  • Graffeo AP and Karger BL (1976) Clin Chem 22(2): 184–187

    Google Scholar 

  • Hay CA, Anderson LA, Roberts MF and Phillipson JD (1985) J Pharm Pharmac, in press

  • Hunter CS, McCalley DV and Barraclough AJ (1982) In: Fujiwara A (ed) Plant Tissue Culture, Japanese

  • Association for Plant Tissue Culture, Tokyo, pp317–308

  • Koblitz H, Koblitz D, Schmauder H-P and Groger D (1983) Plant Cell Reports 2: 122–125

    Google Scholar 

  • Krueger, RJ and Carew DP (1978) Lloydia 41(4): 327–331

    Google Scholar 

  • Misawa M (1985) In: Fiechter A (ed) Advances in Biochemical Engineering/Biotechnology 31, Springer-Verlag, Berlin, Heidelberg, New York and Tokyo, pp59–88

    Google Scholar 

  • Mulder-Krieger Th, Verpoorte R and Baerheim-Svendsen A (1982a) Planta Med 44: 237–240

    Google Scholar 

  • Mulder-Krieger Th, Verpoorte R, de Graaf YP, van der Kreek M and Baerheim-Svendsen A (1982b) Planta Med 46: 15–18

    Google Scholar 

  • Mulder-Krieger Th, Verpoorte R, De Water A, van Gessel M, van Oeveren BCJA and Baerheim-Svendsen A (1982c) Planta Med 46: 19–24

    Google Scholar 

  • Mulder-Krieger Th, Verpoorte R, van der Kreek M and A Baerheim-Svendsen (1984) Planta Med 50: 17–20

    Google Scholar 

  • Smith E (1984) J Chromatog 299: 233–244

    Google Scholar 

  • Staba EJ and Chung AC (1981) Phytochemistry 20(11): 2495–2498

    Google Scholar 

  • Veliky IA (1972) Phytochemistry 11: 1405–1406

    Google Scholar 

  • Veliky IA and Barber KM (1975) Lloydia 38: 125–130

    Google Scholar 

  • Wijnsma R, Verpoorte R, Mulder-Krieger Th and Baerheim-Svendsen A (1984) Phytochemistry 23(10): 2307–2311

    Google Scholar 

  • Zenk MH, El-Shagi H, Arens H, Stockigt J, Weiler EW and Deus B (1977) in: Barz W, Reinhard E and Zenk MH (eds) Plant Tissue Culture and its Bio-technological Application, Springer-Verlag, Berlin, Heidelberg and New York pp27–43

    Google Scholar 

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Communicated by M.H.Zenk

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Hay, C.A., Anderson, L.A., Roberts, M.F. et al. In vitro cultures of Cinchona species. Plant Cell Reports 5, 1–4 (1986). https://doi.org/10.1007/BF00269704

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  • DOI: https://doi.org/10.1007/BF00269704

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