Skip to main content
SpringerLink
Log in

ATPase activities in partially purified membranes of Acetabularia

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Partly purified membranes (with plasmalemma material) of Acetabularia mediterranea were studied with respect to ATPase activity in alkali- and Ca++-free media and its sensitivity to pH (5 – 9), oligomycin (200 ώg/mg protein), 100 ώM N-N′-dicyclohexylcarbodiimide (DCCD), and 50 ώM vanadate. Besides activities which may originate from mitochondrial H+ ATPase (oligomycin-sensitive, alkaline pH optimum) and tonoplast H+ ATPase (DCCD-sensitive, pH optimum 7.5), there is ATPase activity with a pH optimum around pH 6.5, sensitive to vanadate and insensitive to DCCD. These results strongly suggest that the electrogenic Cl− pump in the plasmalemma of Acetabularia is an ATPase. Effects of Mg++, Mg-ATP, ADP, GTP, UTP, CTP and HCO3 − versus Cl− on this ATPase activity are described.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bowman BJ, Blasco F, Allen KE, Slayman CW (1980) In: Spanswick RM, Lucas WL, Dainty J (eds) Plant Membrane Transport: Current Conceptual Issues, Elsevier/North-Holland Biomedical Press, Amsterdam, pp 195–205

    Google Scholar 

  • Bowman EJ (1981) XIII International Botanical Congress, Sydney, Abstracts 01 08 56

  • Cambraia J, Hodges TK (1980) In: Spanswick RM, Lucas WL, Dainty J (eds) Plant Membrane Transport: Current Conceptual Issues, Elsevier/North-Holland Biomedical Press, Amsterdam, pp 211–222

    Google Scholar 

  • DePont JJHHM, Bonting SL (1981) In: Bonting SL, PePont JJHHM (eds) Membrane Transport, Elsevier/North-Holland Biomedical Press, Amsterdam, pp 208–234

    Google Scholar 

  • Gallagher SR, Leonard RT (1982) Pl Physiol 70: 1335–1340

    Google Scholar 

  • Gradmann D (1975) J Membrane Biol 25: 183–208

    Google Scholar 

  • Gradmann D, Tittor J, Goldfarb V (1982) Phil Trans R Soc Lond B 299: 447–457

    Google Scholar 

  • Keck K (1964) In: Prescott DM (ed) Methods in Cell Physiology 1, Academic Press, New York, pp 189–213

    Google Scholar 

  • Kakinuma Y, Ohsumi Y, Anraku Y (1981). J Biol Chem 256: 10859–10863

    Google Scholar 

  • Lanzetta PA, Alvarez LJ, Reinach PS, Candia OA (1979) Anal Biochem 100: 95–97

    Google Scholar 

  • Legros F, Uytenhoef P, Dumont I, Hanson B, Jeanmart J, Massant B, Conrad V (1975) Protoplasma 86: 119–134

    Google Scholar 

  • McKnight GS (1977) Anal Biochem 78: 86–92

    Google Scholar 

  • Perlin DS, Spanswick RM (1980) Pl Physiol 78: 86–92

    Google Scholar 

  • Wallach DFM, Soderberg J, Bricker L (1960) Cancer Research 20: 397–402

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max-Planck-Institut für Biochemie, D-8033, Martinsried, FRG

    V. Goldfarb & D. Gradmann

Authors
  1. V. Goldfarb
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Gradmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by P. Matile

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goldfarb, V., Gradmann, D. ATPase activities in partially purified membranes of Acetabularia . Plant Cell Reports 2, 152–155 (1983). https://doi.org/10.1007/BF00269342

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269342

Keywords

Search

Navigation