Abstract
Protoplasts preparated from dark grown seedlings of Pinus sylvestris L. were incubated with 3-indole (1-14C) acetic acid and 3-indole (2-14C) acetic acid (IAA). Three catabolites were consistently produced in the (2-14C) IAA feeds, one of which co-chromatographed with 3-indole methanol on reversed phase high performance liquid chromatography (HPLC). Protoplasts feed with (1-14C) IAA produced only one labelled catabolite. The non-decarboxylated compound formed was highly polar on reversed phase HPLC, both in the ion suppression and the ion pair mode. The substance was not hydrolysable at pH 11 and 100° indicating that it is not a conjugated form. Effects of time of incubation, pH and the cofactors hydrogen peroxide and 2,4-dichlorphenol on the catabolic rate of IAA are discussed.
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Abbreviations
- BSA:
-
bovine serum albumin
- DCP:
-
2,4-dichlorophenol
- HPLC:
-
high performance liquid chromatography
- IAA:
-
3-indole acetic acid
- IAld:
-
3-indole carboxaldehyde
- ICA:
-
3-indole carboxylic acid
- IM:
-
3-indole methanol
- MES:
-
4-morpholineethane sulfonic acid
- MO:
-
3-methyl-oxindol
- MnO:
-
3-methylene-oxidol
- OxIAA:
-
3-oxindole acetic acid
- PEG:
-
polyethylene glycol
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Communicated by D. von Wettstein
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Sandberg, G., Hällgren, J.E. Catabolism of 3-indole acetic acid in protoplasts from etiolated seedlings of scots pine (Pinus sylvestris L.). Plant Cell Reports 4, 100–103 (1985). https://doi.org/10.1007/BF00269217
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DOI: https://doi.org/10.1007/BF00269217