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Plant regeneration from leaf explants of Glycine clandestina Wendl

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Abstract

Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the same G. clandestina accession. We were not successful in regenerating plants from root tissue of G. clandestina.

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Abbreviations

6-BA:

6-Benzyladenine

FAA:

Formalin

NAA:

Naphthalenacetic acid

IAA:

Indoleacetic acid

Na2EDTA:

Disodium salt Ethylenediamine tetraacetic acid

Fe-NaEDTA:

Ferric-Sodium salt Ethylenediamine tetraacetic acid

References

  • Beversdorf WD, Bingham ET (1977) Crop Sci 17:307–311

    Google Scholar 

  • Cheng TY, Saka H, Voqui-Dinh TH (1980) Plant Sci Lett 19:91–99

    Google Scholar 

  • Christianson ML, Warnick DA, Carlson PS (1983) Science 222:632–634

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50:151–158

    Google Scholar 

  • Grant JE (1984) Plant Cell Tissue and Organ Culture 3:169–173

    Google Scholar 

  • Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Robers SG, Fraley RT (1985) Science 227:1229–1231

    Google Scholar 

  • Johansen DA (1940) Plant Microtechnique 1st Edition McGraw-Hill Book Co. Inc., New York

  • Kameya T, Widholm JM (1981) Plant Sci Lett 21:289–294

    Google Scholar 

  • Kartha KK (1975) In Plant Tissue Culture Methods, Gamborg OL, Wetter LR (Eds.) National Research Council, Saskatoon Canada pp. 44–49

    Google Scholar 

  • Newell CA Hymowitz T (1982) Crop Sci 22:1062–1065

    Google Scholar 

  • Newell CA, Luu HT (1985) Plant Cell Tissue and Organ Culture 4:145–149

    Google Scholar 

  • O'Brien TP, McCully ME (1981) The Study of Plant Structure Principles and Selected Methods Termarcarphi Pty. Ltd., Melbourne, Australia

  • Singh RJ, Hymowitz T (1985a) Euphytica 34:187–192

    Google Scholar 

  • Singh, RJ, Hymowitz T. (1985b) Theor Appl Genet 71:221–230

    Google Scholar 

  • Vaughan DA, Hymowitz T (1983) Plant Genetic Resources Newsletter No. 56:7–12

  • White PR (1963) The Cultivation of Animal and Plant Cells 2nd Edition Ronald Press, New York

  • Widholm JM, Rick S (1983) Plant Cell Reports 2:19–20

    Google Scholar 

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Authors and Affiliations

  1. Department of Agronomy, University of Illinois, 61801, Urbana, IL, USA

    T. Hymowitz, N. L. Chalmers, S. H. Costanza & M. M. Saam

Authors
  1. T. Hymowitz
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  2. N. L. Chalmers
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  3. S. H. Costanza
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  4. M. M. Saam
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Additional information

Communicated by O. Gamborg

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Hymowitz, T., Chalmers, N.L., Costanza, S.H. et al. Plant regeneration from leaf explants of Glycine clandestina Wendl. Plant Cell Reports 5, 192–194 (1986). https://doi.org/10.1007/BF00269116

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  • DOI: https://doi.org/10.1007/BF00269116

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