Summary
l-Ureidosuccinic acid (USA), an intermediate metabolite of pyrimidine biosynthesis, inhibits cell growth. Mutants blocked in pyrimidine biosynthesis after dihydroorotate (DHO), are more resistant to USA than are the wild type and the mutants blocked in the synthesis of DHO. This difference in sensitivity correlates with the level of dihydroorotase activity. Purines revert USA inhibition. Mutants selected for their resistance to USA were resistant because of a) overproducing purines (adenine excretion), b) having a reduced USA uptake, or c) showing a USA resistance independant of the above phenotypes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bach, M. L., Lacroute, F.: Direct selective techniques for the isolation of pyrimidine auxotrophs in yeast. Molec. gen. Genet. 115, 126–130 (1972)
Burns, V. W.: Regulation of pyrimidine biosynthesis and its strong coupling to the purine system. Biophys. J. 6, 787–800 (1966)
De Robichon-Szulmajster, H., Surdin-Kerjan, Y.: Nucleic acid and protein synthesis in yeast: Regulation of synthesis and activity. The yeasts II, nineth edit., p. 336–418, New York-London: Academic Press (1971)
Drillien, R.: Etude Biochimique et génétique de mutations qui modifient la perméation de l'acide uréidosuccinique et le métabolisme de l'azote chez la levure Saccharomyces cerevisiae. Thèse de doctorat de troisième cycle, Université Louis Pasteur, Strasbourg, France (1972)
Drillien, R., Lacroute, F.: Uréidosuccinic acid uptake in yeast and some aspects of its regulation. J. Bact. 109, 203–208 (1972)
Gerhart, J. C., Pardee, A. B.: The enzymology of control of feedback inhibition. J. Biol. Chem. 237, 891–896 (1962)
Grenson, M., Hou, C., Grabeel, M.: Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. IV. Evidence for a general amino acid permease. J. Bact. 103, 770–777 (1970)
Gross, T. S., Woods, R. A.: Identification of mutants defective in the first and second steps of de novo purine synthesis in Saccharomyces cerevisiae. Biochim. biophys. Acta (Amst.) 247, 13–21 (1971)
Jund, R., Lacroute, F.: Regulation of ortidylic acid pyrophosphorylase in Saccharomyces cerevisiae. J. Bact. 109, 196–202 (1972)
Kaplan, J. G., Duphil, M., Lacroute, F.: A study of aspartate transcarbamylase activity of yeast. Arch. Biochem. Biophys. 119, 541–551 (1967)
Lacroute, F.: Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae. J. Bact. 95, 824–832 (1968)
Lacroute, F.: Abstracts Proceedings of 7th FEBS meeting. Varna (Bulgaria): 303
Lomax, C. A., Woods, R.A.: A complex genetic locus controlling purine nucleotide biosynthesis in yeast. Molec. gen. Genet. 120, 139–149 (1973)
Martin, D.W.: Radioassay for enzymic production of glutamate from glutamine. Analyt. Biochem. 46, 239–243 (1972)
Mortimer, R. K., Hawthorne, D. C.: Genetic mapping in Saccharomyces cerevisiae. Genetics 58, 165–173 (1966)
Author information
Authors and Affiliations
Additional information
Communicated by F. Gros
Rights and permissions
About this article
Cite this article
Korch, C.T., Lacroute, F. & Exinger, F. A regulatory interaction between pyrimidine and purine biosyntheses via ureidosuccinic acid. Molec. Gen. Genet. 133, 63–75 (1974). https://doi.org/10.1007/BF00268678
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00268678