Summary
KR103 is a kanamycin resistant cell line of Nicotiana sylvestris, deficient in inducible shoot redifferentiation. The existence of a genetically unaltered inducibility factor in KR103 was tested by fusing protoplasts of this line with those of Nicotiana knightiana, which can not be induced to form shoots in callus culture.
Selection of somatic hybrids was based on screening for kanamycin resistance and green pigmentation, factors originally separated in the parental lines. The hybrid nature of two lines (H1 and H2) was shown by studies on esterase, alcohol dehydrogenase and glucose-6-phosphate dehydrogenase isoenzymes.
In the somatic hybrids shoot formation on inductive medium was restored, indicating that the inducibility factor had not been altered in KR103. Genetic complementation and induced regulatory changes due to genome interaction are discussed as possible explanations for the restoration of morphogenic potential.
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Maliga, P., Lázár, G., Joó, F. et al. Restoration of morphogenic potential in Nicotiana by somatic hybridisation. Molec. Gen. Genet. 157, 291–296 (1977). https://doi.org/10.1007/BF00268665
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DOI: https://doi.org/10.1007/BF00268665