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Transfection of Escherichia coli by Mu DNA

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Summary

Infectivity of Mu DNA was demonstrated in Ca++-treated Escherichia coli cells that lacked the nucleases Exo V and Endo I. The efficiency of transfection is about 10-7 per phage equivalent. Infectivity is destroyed by denaturation of Mu DNA, and cannot be restored by renaturation.

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Authors and Affiliations

  1. Cold Spring Harbor Laboratory, 11724, Cold Spring Harbor, New York, U.S.A.

    R. Kahmann, D. Kamp & D. Zipser

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  1. R. Kahmann
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  2. D. Kamp
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  3. D. Zipser
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Communicated by E. Bautz

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Kahmann, R., Kamp, D. & Zipser, D. Transfection of Escherichia coli by Mu DNA. Molec. Gen. Genet. 149, 323–328 (1976). https://doi.org/10.1007/BF00268534

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  • DOI: https://doi.org/10.1007/BF00268534

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