Summary
Fragments of λdrif d 18 DNA with different end-points within the set of structural genes of ribosomal proteins L11 (rplK), L1 (rplA), L10 (rplJ) and L12 (rplL) as well as the β (rpoB) and β′ (rpoC) subunits of RNA polymerase have been cloned on plasmids. These plasmids were transformed in host cells which were mutant for each of the genes, enabling expression of both wild-type (plasmid-borne) and mutant (chromosomal) genes to be differentiated. On the basis of these results we propose the following genetic structure for the region: rplK and rplA are in one operon; rplL, rpoB and rpoC are in a second. Our data suggest the possibility that rplJ is by itself in an operon situated between the other two.
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Anderson, E.H.: Growth requirements of virus resistant Escherichia coli strain B. Proc. Natl. Acad. Sci. U.S.A. 32, 120–122 (1946)
Bachmann, B.J., Low, B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bacteriol. Rev. 40, 116–167 (1976)
Bendiak, D.S., Parker, J., Friesen, J.D.: Fine structure mapping of the rts, rplK, rplL and rpoB genes of Escherichia coli. J. Bacteriol. 129, 536–539 (1977)
Blattner, F.R., Dahlberg, J.E., Boettiger, J.K., Fiandt, M., Szybalski, W.: Distance from a promoter mutation to an RNA synthesis startpoint on bacteriophage DNA. Nature New Biol. 237, 232–236 (1972)
Bolivar, F., Rodriguez, R.L., Greene, B.J., Betlach, M.C., Heyneker, H.L., Boyer, H.W., Crosa, J.H., Falkow, S.: The circular restriction map of pBR322. In: DNA insertion elements, plasmids and episomes (Bukhari, J.A., Shapiro, J.A., Adhya, S.L., eds.). Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory (1977)
Bronson, M.J., Squires, C., Yanofsky, C.: Nucleotide sequences from trystophan messenger RNA of Escherichia coli: the sequence corresponding to the amino-terminal region of the first polypeptide specified by the operon. Proc. Natl. Acad. Sci. U.S.A. 70, 2335–2339 (1973)
Clewell, O.B., Helinski, D.R.: Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry 9, 4428–4440 (1970)
Collins, J., Fiil, N.P., Jørgensen, P., Friesen, J.D.: Gene cloning of Escherichia coli chromosomal genes important in the regulation of ribosomal RNA synthesis. In: Control of ribosome RNA synthesis (Kjelgaard, N.O. and Maaløe, O., eds.). Copenhagen: Munksgaard 1976
Dabbs, E.R., Wittmann, H.G.: A strain of Escherichia coli which gives rise to mutations in a large number of ribosomal proteins. Mol. Gen. Genet. 149, 303–309 (1976)
Dennis, P.P., Nomura, M.: Stringent control of ribosomal protein gene expression in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 71, 3819–3823 (1974)
Friesen, J.D., Fiil, N.P., Parker, J., Haseltine, W.A.: A new relaxed mutant of Escherichia coli with an altered 50S ribosomal subunit. Proc. Natl. Acad. Sci. U.S.A. 71, 3465–3469 (1974)
Furano, A.V., Wittel, F.P.: Syntheses of elongation factors Tu and G are under stringent control in Escherichia coli. J. Biol. Chem. 251, 898–901 (1976a)
Furano, A.V., Wittel, F.P.: Effect of the relA gene on the synthesis of individual proteins in vivo. Cell 8, 115–122 (1976b)
Hardy, S.J.S.: The stoichiometry of the ribosomal proteins L7, L10, L11 and L12 on the 50S subunit of Escherichia coli. J. Biol. Chem. 250, 831–834 (1975)
Isono, K., Krauss, J., Hirota, Y.: Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins. Mol. Gen. Genet. 149, 297–302 (1976)
Kaltschmidt, E., Wittmann, H.G.: Ribosomal proteins VII. Two dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal. Biochem. 36, 401–412 (1970)
Kirschbaum, J.B., Konrad, E.B.: Isolation of a specialized transducing bacteriophage carrying the beta subunit for Escherichia coli ribonucleic acid polymerase. J. Bacteriol. 116, 517–526 (1973)
Kirschbaum, J.B., Scaife, J.: Evidence for a λ transducing phage carrying the genes for β and β′ subunits of Escherichia coli RNA polymerase. Mol. Gen. Genet. 132, 193–201 (1974)
Li, K., Subramanian, A.R.: Selective separation procedure for determination of ribosomal proteins L7 and L12. Anal. Biochem. 64, 121–129 (1975)
Lindahl, L., Jaskunas, S.R., Dennis, P.P., Nomura, M.: Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA and RNA polymerase subunits. Proc. Natl. Acad. Sci. U.S.A. 72, 2743–2747 (1975)
Lindahl, L., Yamamoto, M., Nomura, M., Kirschbaum, J., Allet, B., Rochaix, J.-D.: Mapping of a cluster of genes for components of the transcriptional and translational machineries of Escherichia coli. J. Mol. Biol. 109, 23–47 (1977)
Linn, T., Scaife, J.: Identification of a single promoter in Escherichia coli for rplJ, rplL and rpoBC. Nature 276, 33–37 (1977)
Mandel, M., Higa, A.: Calcium dependent bacteriophage DNA injection. J. Mol. Biol. 53, 159–162 (1970)
Miller, J.H.: Experiments in molecular genetics. Cold Spring Harbor, N.Y.: Cold Spring Harbor laboratory 1972
Parker, J., Watson, R.J., Friesen, J.D., Fiil, N.P.: A relaxed mutant with an altered ribosomal protein L11. Mol. Gen. Genet. 144, 111–114 (1976)
Pedersen, S., Bloch, P.L., Reeh, S., Neidhart, F.C.: Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates. Cell 14, 179–190 (1978)
Pedersen, S., Reeh, S.V., Parker, J., Watson, R.J., Friesen, J.D., Fiil, N.F.: Analysis of the proteins synthesized in ultraviolet light-irradiated Escherichia coli following injection with the bacteriophage λdrif d 18 and λdfus-3. Mol. Gen. Genet. 144, 339–344 (1976)
Reeh, S., Pedersen, S., Friesen, J.D.: Biosynthetic regulation of individual proteins in relA + and relA strains of Escherichia coli during amino acid starvation. Mol. Gen. Genet. 149, 279–289 (1976)
Scaife, J.: Bacterial RNA polymerases, the genetics and control of their synthesis. In: RNA polymerase (Chamberlin, M. and Losick, R., eds.). Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1976
Sharp, P.A., Sugden, B., Sambrook, J.: Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis. Biochemistry 12, 3055–3063 (1973)
Subramanian, A.R.: Copies of L7 and L12 and heterogeneity of the large subunit of Escherichia coli ribosome. J. Mol. Biol. 95, 1–8 (1975)
Tanaka, F., Weisblum, B.: Construction of a colicin El-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J. Bacteriol. 121, 354–362 (1975)
Watson, R.J., Parker, J., Fiil, N.P., Flaks, J.G., Friesen, J.D.: New chromosomal location for structural genes of ribosomal proteins. Proc. Natl. Acad. Sci. U.S.A. 72, 2765–2769 (1975)
Weber, H.J.: Stoichiometric measurements of 30S and 50S ribosomal proteins from Escherichia coli. Mol. Gen. Genet. 119, 233–248 (1972)
Yamamoto, M., Nomura, M.: Cotranscription of genes for RNA polymerase subunits β and β′ with genes for ribosomal proteins in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 75, 3891–3895 (1978)
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Communicated by H.G. Wittmann
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Fiil, N.P., Bendiak, D., Collins, J. et al. Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids. Molec. Gen. Genet. 173, 39–50 (1979). https://doi.org/10.1007/BF00267689
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DOI: https://doi.org/10.1007/BF00267689