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Genetic studies of the ribosomal proteins in Escherichia coli XI

Mapping of the genes for L21, L27, S15 and S21 by using hybrid bacteria and over-production of these proteins in the merodiploid strains

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Summary

E. coli episomes which cover argG region were transferred to S. marcescens and ribosomal proteins (r-proteins) from these hybrid strains were analyzed by two-dimensional (2D) polyacrylamide gel electrophoresis. Three E. coli r-proteins L21, L27 and S15 could be detected in the ribosomes from the hybrid strains.

The relative rate of synthesis of the individual r-proteins were determined for E. coli merodiploid strains harboring these episomes. Over-production of three r-proteins L21, L27 and S21 (but not S15) was observed in the merodiploid strains.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Kasumi-cho, Hiroshima, Japan

    Renkichi Takata

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  1. Renkichi Takata
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Communicated by H.G. Wittmann

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Takata, R. Genetic studies of the ribosomal proteins in Escherichia coli XI. Molec. Gen. Genet. 160, 151–155 (1978). https://doi.org/10.1007/BF00267476

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  • DOI: https://doi.org/10.1007/BF00267476

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