Summary
We have studied the in vitro properties of ribosomes from several mutants resistant to erythromycin. Mutations in three different genes may confer resistance to erythromycin. Two of them are structural genes for proteins L4 and L22 of the large subunit. The third mutation (in eryC gene) seems to affect mainly the small subunit. The mechanism of action of the antibiotic may involve both subunits.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bachman, J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K12. Bact. Rev. 40, 116–167 (1976)
Debey, P., Hui Bon Hoa, G., Douzou, P., Godefroy-Colburn, T., Graffe, M., Grundberg-Manago, M.: Ribosomal subunit interaction a studied by light scattering. Evidence of different classes of ribosomes preparations. Biochem. 14, 1553–1559 (1975)
Hardy, S.J.S., Kurland, C.G., Yoynow, P., Mora, G.: The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochem. 8, 2897–2905 (1969)
Kaltschmidt, E., Wittmann, H.G.: Ribosomal proteins. VII. Two dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Ann. Biochem. 36, 401–412 (1970)
Kurland, C.G.: The requirement for specific sRNA binding by ribosomes. J. molec. Biol. 18, 90–108 (1966)
Leder, P., Bursztyn, H.: Initiation of protein synthesis. A convenient assay for the ribosome-dependent synthesis of N-formyl-14C-methionyl puromycin. Biochem. biophys. Res. Commun. 25, 233–238 (1966)
Oleinick, N.L.: The erythromycin. In: Antibiotics III (J.N. Corcoran and F.E. Hahn, eds.), pp. 396–419. Berlin-Heidelberg-New York: Springer 1975
Oleinick, N.L., Corcoran, J.W.: Two types of binding of erythromycin to ribosomes from antibiotic-sensitive and resistant Bacillus subunit 168. J. biol. Chem. 244, 727–735 (1969)
Otaka, E., Teraoka, H., Tamaki, M., Tanaka, K., Osawa, S.: Ribosomes from erythromycin-resistant mutants of Escherichia coli Q13. J. molec. Biol. 48, 499–510 (1970)
Pardo, D., Rosset, R.: Genetic studies of erythromycin resistant mutants of Escherichia coli. Molec. gen. Genet. 135, 257–268 (1974)
Pardo, D., Rosset, R.: A new ribosomal mutations which affects the two ribosomal subunits in Escherichia coli. Molec. gen. Genet. 153, 199–204 (1977)
Pestka, S.: Binding of 14C-erythromycin to Escherichia coli ribosomes. Antimicrob. Agents and Chemist. 6, 474–478 (1974)
Saltzman, L., Apirion, D.: Binding of erythromycin to the 50S ribosomal subunit is affected by alterations in the 30S ribosomal subunit. Molec. gen. Genet. 143, 301–306 (1976)
Traub, P., Mizushima, S., Lowry, C.V., Nomura, M.: Reconstitution of ribosomes from subribosomal components. In: Methods in enzymology (Moldave, K. and Grossman, L. eds.), Vol. XX, pp. 391–407. New York-London: Academic Press 1971
Wittmann, H., Stöffler, G., Apirion, D., Rosen, L., Tanaka, K., Tamaki, M., Takata, R., Dekio, S., Otaka, E., Osawa, S.: Biochemical and genetic studies on two different types of erythromycin resistant mutants of Escherichia coli with altered ribosomal proteins. Molec. gen. Genet. 127, 175–189 (1973)
Author information
Authors and Affiliations
Additional information
Communicated by H.G. Wittmann
Rights and permissions
About this article
Cite this article
Pardo, D., Rosset, R. Properties of ribosomes from erythromycin resistant mutants of Escherichia coli . Molec. Gen. Genet. 156, 267–271 (1977). https://doi.org/10.1007/BF00267181
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00267181