Summary
Genetic analyses demonstrate the segregation of three targets for the K restriction system in h 80 i λ hybrid phages. Mutations in each of these three targets have been isolated and shown to confer resistance in cis but not in trans. Two of the three targets (sk-1 and sk-2) have been located on the λ genome: sk-1 is right of gene R and sk-2 is between genes cIII and N. The third target is in the phi80 genome right of, but close to, att. Phage λ lacking both sk-1 and sk-2 retains at least 3 targets for the K restriction system.
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Communicated by F. Stahl
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Murray, N.E., de Ritis, P.M. & Foster, L.A. DNA targets for the Escherichia coli K restriction system analysed genetically in recombinants between phages Phi80 and lambda. Molec. Gen. Genet. 120, 261–281 (1973). https://doi.org/10.1007/BF00267157
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DOI: https://doi.org/10.1007/BF00267157