Summary
A method for selective staining of flavan-3-ols in plant tissues fixed with glutaraldehyde is given. The use of glycolmethacrylate as embedding medium allows the sulphuric acid-containing staining solution to be heated without destroying the fine structure of the tissue. The distribution of flavan-3-ols and proanthocyanidins in different plant tissues is discussed.
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Gutmann, M., Feucht, W. A new method for selective localization of flavan-3-ols in plant tissues involving glycolmethacrylate embedding and microwave irradiation. Histochemistry 96, 83–86 (1991). https://doi.org/10.1007/BF00266765
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DOI: https://doi.org/10.1007/BF00266765