Summary
A thermodynamic model of particle adhesion from a suspension onto a solid surface is used to predict the extent of adhesion of suspension-cultured Catharanthus roseus cells to the following polymer substrates: fluorinated ethylene-propylene (FEP), polystyrene (PS), polyethylene terephthalate (PET), sulphonated polystyrene (SPS), and glass. According to this model, the extent of adhesion is determined by the surface tensions of the plant cells, the polymer substrates, and the suspending liquid medium. Experimentally, adhesion of the washed plant cells was found to decrease with increasing substrate surface tension, following the sequence FEP>PS>PET>SPS>glass, when the surface tension of the liquid was greater than that of the plant cells, in agreement with the model. However, adhesion increased with increasing substrate surface tension when the liquid surface tension was lower than the cellular surface tension, also in agreement with the model. When the liquid and cellular tensions were equal the extent of adhesion was independent of the substrate surface tension. This also agrees with model predictions and leads to a value for the surface tension of C. roseus cells of approximately 54 ergs/cm2 which is in agreement with a value obtained from contact angle measurements on layers of cells and sedimentation volume analysis. The cellular surface tension determined by the sedimentation volume method showed a biphasic alteration during growth cycles of C. roseus cell cultures. These variations (between 55 and 58 ergs/cm2) agree with the pattern of adhesion previously described.
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Absolom DR, Neumann AW, Zingg W, van Oss CJ (1979) Thermodynamics of cell adhesion. Trans Am Soc Artif Intern Organs 25:152–158
Absolom DR, Francis DW, Zingg W, van Oss CJ, Neumann AW (1982) Platelet phagocytosis of bacteria: surface thermodynamic aspects. J Colloid Interface Sci 85:168–177
Absolom DR, Lamberti FV, Policova Z, Zingg W, van Oss CJ, Neumann AW (1983) Surface thermodynamics of bacterial adhesion. J Appl Environ Microbiol 46:90–97
Absolom DR, Zingg W, Neumann AW (1986) Measurement of contact angles on biological and other highly hydrated surfaces. J Colloid Interface Sci 112:599–601
Absolom DR, Policova Z, Bruck T, Thomson C, Zingg W, Neumann AW (1987) Surface characterization of protein-coated polymer surfaces by means of the sedimentation volume method. J Colloid Interface Sci 117:550–564
Atkinson B, Fowler HW (1974) The significance of microbial films in fermenters. Adv Biochem Eng 3:221–227
Brodelius P, Mosbach K (1982) Immobilized plant cells, Advances in applied microbiology 28:1–26
Characklis WG (1973) Attached microbial growths I. Attachment and growth. Water Res 7:1113–1127
Facchini PJ, Radvanyi LG, Gigeure Y, DiCosmo F (1988) Adhesion of Catharanthus roseus cells to polymer surfaces: effect of substrate hydrophobicity. Biotech Bioengineer (in press)
Fletcher M (1980) The question of passive versus active attachment mechanisms in non-specific bacterial adhesion. In: Berkeley RCW, Lynch JM, Melling J, Rutter PR, Vincent B (eds) Microbial adhesion to surfaces. Ellis Horwood, London, p 197
Furuya T, Yoshikawa T, Taira M (1984) Biotransformation of codeinone to codeine by immobilized cells of Papaver somniferum. Phytochemistry 23:999–1002
Lindsey K, Yeoman MM (1985) Immobilized plant cells. In: Yeoman MM (ed) Plant cell culture technology. Blackwell Scientific Publications, Oxford, p 226
Marshall KC, Stout R, Mitchell R (1971) Selective sorption of bacteria from sea water. Can J Microbiol 17:1413–1416
Murashige TO, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15:473–497
Neumann AW, Good RJ, Ehrlich P, Basu PK, Johnston BJ (1973) The temperature dependence of the surface tension of solutions of atactic polystyrene. J Macromol Sci Phys 7:525–529
Neumann AW, Good RJ, Hope CJ, Sejpal M (1974) An Equation-of-state approach to determine surface tensions of low-energy solids from contact angles. J Colloid Interface Sci 49:291–304
Neumann AW, Absolom DR, Zingg W, van Oss CJ (1979a) Surface thermodynamics of leukocyte and platelet adhesion to polymer surfaces. Cell Biophys 1:79–92
Neumann AW, Good RJ (1979b) Techniques of measuring contact angles. In: Good RJ, Stromberg (eds) Surface and Colloid Science II: Experimental Methods. Plenum Press, New York, p 31
Neumann AW, Hum OS, Francis DW, Zingg W, van Oss CJ (1980a) Kinetic and thermodynamic aspects of platelet adhesion from suspension to various substrates. J Biomed Mater Res 14:499–509
Neumann AW, Absolom DR, Francis DW, van Oss CJ (1980b) Conversion tables of contact angles to surface tensions. Sep Purif Methods 9:62–163
Nguyen T, John's WE (1970) Polar and dispersion force contributions to the total surface free energy of wood. Wood Sci Technol 12:63–74
Robins RJ, Hall DO, Shi DJ, Turner RJ, Rhodes MJC (1986) Mucilage acts to adhere cyanobacteria and cultured plant cells to biological and inert surfaces. FEMS Microbiol Lett 34:155–160
Rosan B, Appelbaum B, Holt SC (1981) Isolation and identification of the surface receptor of Streptococcus sanguis responsible for adherence to hydroxyapatite. In: Lynch JM, Melling J, Rutter PR, Vincent B (eds) Microbial Adhesion to Surfaces. Halstead Press, New York, p 537
Tanaka H (1981) Technological problems in cultivation of plant cells at high density. Biotech Bioengineer 23:1203–1218
Taylor JA, West DW (1980) The use of evans's blue stain to test the survival of plant cells after exposure to high salt and osmotic pressure. J Exp Bot 31:571–576
Trasher SG, Yoshida T, van Oss CJ, Cohen S, Rose NR (1973) Alteration of macrophage interfacial tension by supernatants of antigen-activated lumpyhocyte cultures. J Immunol 110:321–326
van Oss CJ, Gillman CF, Neumann AW (1975) Phagocytic engulfment and cell adhesiveness as surface phenomena, Isenberg, H (ed) Marcel Dekker, New York
van Oss CJ, Absolom DR, Neumann AW, Zingg W (1981) Determination of the surface tension of proteins. I. Surface tension of native serum proteins in aqueous media. Biochem Biophys Acta 670:64–73
Vargh-Butler EI, Zubovitz TK, Hamza HA, Neumann AW (1985a) Surface tension effects in the sedimentation of polymer particles in various liquids. J Dispersion Sci Technol 6:357–379
Vargha-Butler EI, Zubovitz TK, Absolom DR, Hamza HA, Neumann AW (1985b) Surface tension effects in the sedimentation of coal particles in various liquids. Chem Eng Commun 33:225–276
Vargha-Butler EI, Zubovitz TK, Weibel MK, Absolom DR, Neumann AW (1985c) Surface tension effects in the sedimentation of coal particles in n-propanol/water mixtures. Colloids Surf 15:233–238
Ward CA, Neumann AW (1974) On the surface thermodynamics of a two component liquid-vapour-ideal solid system. J Colloid Interface Sci 49:286–290
Widholm JM (1972) The Use of fluorescein diacetate and phenosafranin for determining viability of cultured plant cells. Stain Technol 47:189–194
Wilson SB, King PJ, Street HE (1971) Studies of the growth in culture of plant cells. Part 12. A versatile system for the large scale batch or continuous culture of plant cell suspensions. J Exp Bot 22:177–207
Woods DE, Straus DC, Johanson WG, Berry UK, Bass JA (1980) Role of pili of Pseudomonas aeruginosa to mammalian buccal epithelial cells. Infect Immun 29:1146–1151
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Facchini, P.J., Neumann, A.W. & DiCosmo, F. Thermodynamic aspects of plant cell adhesion to polymer surfaces. Appl Microbiol Biotechnol 29, 346–355 (1988). https://doi.org/10.1007/BF00265818
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DOI: https://doi.org/10.1007/BF00265818