Summary
An in situ hybridization procedure was developed for mitotic potato chromosomes by using a potato 24S rDNA probe. This repetitive sequence hybridized to the nucleolar organizer region (NOR) of chromosome 2 in 95%–100% of the metaphase plates. Another repetitive sequence (P5), isolated from the interdihaploid potato HH578, gave a “ladderpattern” in genomic Southern's of Solanum tuberosum and Solanum phureja, but not in those of Solanum brevidens and two Nicotiana species. This sequence hybridized predominantly on telomeric and centromeric regions of all chromosomes, although chromosomes 7, 8, 10 and 11 were not always labeled clearly.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ambros PF, Matzke AJM, Matzke MA (1986a) Localization of Agrobacterium rhizogenes T-DNA in plant chromosomes by in situ hybridization. EMBO J 5:2073–2077
Ambros PF, Matzke MA, Matzke AJM (1986b) Detection of a 17 kb unique sequence (T-DNA) in plant chromosomes by in situ hybridization. Chromosoma 894:11–18
Bedbrook J (1981) A plant DNA preparation method. Plant Mol Biol Newslett II:24
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Cullis CA, Cleary W (1986) Rapidly varying DNA sequences in flax. Can J Genet Cytol 28:252–259
Donlon TA (1986) Practical approaches to in situ hybridization. Karyogram 12:3–10
Gall JG, Pardue ML (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci USA 63:378–383
Hutchinson J, Lonsdale LM (1982) The chromosomal distribution of cloned highly repetitive sequences from hexaploid wheat. Heredity 48:371–376
Jamieson G, Evans IJ, Barnes SR (1986) An enzymic method of preparing plant chromosomes for in situ hybridization. Stain Technol 61:21–25
Landegent JE, Jansen in de Wal N, Baan RA, Hoeijmakers JHJ, v d Ploeg M (1984) 2-Acetylaminofluorene-modified probes for the indirect hybridocytochemical detection of specific nucleic acid sequences. Exp Cell Res 153:61–72
Landsmann J, Uhrig H (1985) Somaclonal variation in Solanum tuberosum detected at the molecular level. Theor Appl Genet 71:500–505
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor NY
Mouras A, Saul MW, Essad S, Potrykus I (1987) Localization by in situ hybridization of a low copy chimaeric resistance gene introduced into plants by direct gene transfer. Mol Gen Genet 207:204–209
Pardue ML (1985) Nucleic Acid Hybridization. A practical approach. In: Hames BD, Higgins SJ (eds) IRL Press, Oxford Washington/DC, pp 170–203
Pijnacker LP, Ferwerda MA (1984) Giemsa C-banding of potato chromosomes. Can J Genet Cytol 26:415–419
Pijnacker LP, Walch K, Ferwerda MA (1986) Behaviour of chromosomes in potato leaf tissue cultured in vitro as studied by BrdC-Giemsa labelling. Theor Appl Genet 72:833–839
Pijnacker LP, Ferwerda MA (1987) Karyotype variation in amino-ethyl-cysteine resistant cell and callus cultures and regenerated plants of a dihaploid potato (Solanum tuberosum). Plant Cell Rep 6:385–388
Ramanna MS, Wagenvoort M (1976) Identification of the trisomic series in diploid Solanum tuberosum L., group tuberosum. I. Chromosome identification. Euphytica 25:233–240
Rayburn AL, Gill BS (1985) Use of biotin-labeled probes to map specific DNA sequences on wheat chromosomes. J Hered 76:78–81
Tautz D, Renz M (1983) An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels. Anal Biochem 132:14–19
Viera J, Messing J (1982) The pUC plasmids, an M13mp7 derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
Vrensen GJFM (1970) Some new aspects of efficiency of electron microscopic autoradiography with tritium. J Histochem Cytochem 18:278–290
de Vries SE, Tempelaar MJ (1987) Electrofusion and analysis of potato somatic hybrids. In: Bajaj YPS (ed) Potato. Springer, Berlin Heidelberg New York (Biotechnology in Agriculture and Forestry, vol 3) pp 211–222
Vosman B, Kooistra J, Olijve J, Venema G (1987) Cloning in Escherichia coli of the gene specfying the DNA entry nuclease of Bacillus subtilis. Gene 52:175–183
Yeh BP, Peloquin SJ (1965) Pachytene chromosomes of the potato (Solanum tuberosum, group antigena). Am J Bot 52:1014–1020
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Visser, R.G.F., Hoekstra, R., van der Leij, F.R. et al. In situ hybridization to somatic metaphase chromosomes of potato. Theoret. Appl. Genetics 76, 420–424 (1988). https://doi.org/10.1007/BF00265343
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00265343