Summary
In a yeast mutant (198 D1) exhibiting temperature sensitive DNA replication, incubation at the restrictive temperature influences both nuclear and mitochondrial DNA synthesis but to different degrees, the mitochondrial DNA being less affected. DNA polymerase activities measurable in mitochondria free cell extracts as well as in the extract from isolated mitochondria are found to be unaffected at the restrictive temperature. The level of DNA polymerase in the cell extract is elevated in comparison to the wild type strain. Furthermore, the tight coupling of DNA replication to protein synthesis usually observed in yeast is partly lost in the mutant.
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Communicated by E. Bautz
This work is dedicated to Professor O. Hoffmann-Ostenhof on the occasion of his 60th birthday.
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Wintersberger, U., Hirsch, J. & Fink, A.M. Studies on nuclear and mitochondrial DNA-replication in a temperature-sensitive mutant of Saccharomyces cerevisiae . Molec. Gen. Genet. 131, 291–299 (1974). https://doi.org/10.1007/BF00264860
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DOI: https://doi.org/10.1007/BF00264860