Summary
The molecular nature of lethal and semilethal mutations in the Pgd locus of D. melanogaster coding for 6-phosphogluconate dehydrogenase (6PGD) was studied. All the 11 mutations affect the structural gene of the Pgd locus: 3 semilethal mutations resulted in altered 6PGD molecules with decreased catalytic activities; the rest 8 lethals were “null” alleles characterized by mutant polypeptides capable of reacting with antisera against highly purified 6PGD.
“Null” or low activity alleles for glucose-6-phosphate dehydrogenase induced by ethyl methanesulfonate were shown to be suppressors for the lethal mutations in the Pgd locus.
A monocistronic type of organization of the Pgd locus is suggested taking into account the biochemical mechanism of suppression of the Pgd-lethals and their location in the structural gene coding for 6PGD.
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References
Abrahamson, S., Bender, M.A., Conger, A.D., Wolf, C.: Uniformity of radiation induced mutation rates among different species. Nature (Lond.) 245, 460–462 (1973)
Ananiev, E.V., Barsky, V.E.: DNA content, replication and transcription rate in different sites of the X-chromosome of D. melanogaster. Molec. Biologia (Russ.) 9, 752–760 (1975)
Auerbach, C., Kilbey, B.J.: Mutations in eukaryotes. Ann. Rev. Genet. 5, 163–218 (1971)
Bahn, E.: A suppressor locus for the pyrimidine requiring mutant: rudimentary. Dros. Inform. Service 49, 98 (1972)
Beermann, W.: Chromomeres and genes. In: Results and problems in cell differnetiation, Vol. 4, pp. 1–33. Berlin-Heidelberg-New York: Springer (1973)
Bewley, G.C., Lucchesi, J.C.: Lethal effects of low and “null” activity alleles of 6-phosphogluconate dehydrogenase in D. melanogaster. Genetics 79, 451–457 (1975)
Bishop, J.B., Lee, W.R.: Chromosome breakage in D. melanogaster induced by a monofunctional alkylating agent (EMS). Mutation Res. 21, 327–333 (1973)
Brenner, S.: The genetics of Caenorhabditis elegans. Genetics 77, 71–94 (1974)
Davidson, E.H., Britten, R.J.: Organization, transription, and regulation in the animal genome. Quart. Rev. Biol. 48, 565–613 (1973)
Gelbart, W.M., McCarron, M., Pandey, J., Chovnick, A.: Genetic limits of the xanthine dehydrogenase structural elements within the rosy locus in D. melanogaster. Genetics 78, 869–886 (1974)
Georgiev, G.P.: On the structural organization of operon and the regulation of RNA synthesis in animal, cells. J. theor. Biol. 25, 473–490 (1969)
Gvozdev, V.A., Birstein, V.J., Faizullin, L.Z.: Gene dependent regulation of 6-phosphogluconate dehydrogenase activity of Drosophila melanogaster. Molec. Biologia (Russ.) 6, 876–887 (1970)
Gvozdev, V.A., Gerasimova, T.I., Kogan, G.L., Braslavskaya, O.Yu.: Role of the pentose phosphate pathway in metabolism of Drosophila melanogaster elucidated by mutations affecting glucose-6-phosphate and 6-phosphogluconate dehydrogenases. FEBS Letters 64, 85–88 (1976)
Gvozdev, V.A., Gostimsky., S.A., Gerasimova, T.I., Dubrovskaya, E.S., Braslavskaya, O.Yu.: Fine genetic structure of the 2D3–2F5 region of the X-chromosome of D. melanogaster. Mol. gen. Genet. 141, 269–275 (1975)
Hartman, P.E., Hartman, Z., Stahl, R.C., Ames, B.: Classification and mapping of spontaneous and induced mutations in the histidin operon of Salmonella. Advanc. Genet. 16, 1–34 (1971)
Hartman, P.E., Roth, J.R.: Mechanisms of suppression. Adv. in Genet. 17, 1–106 (1973)
Judd, B.H., Shen, M.W., Kaufman, T.C.: The anatomy and function of a segment of the X-chromosome of D. melanogaster. Genetics 71, 139–156 (1972)
Kazazian, H.H., Young, W.J., Childs, B.: X-linked 6-phosphogluconate dehydrogenase in Drosophila: Subunit association. Science 150, 1601–1602 (1965)
Kazazian, H.H.: Molecular size studies in 6-phosphogluconate dehydrogenase. Nature (Lond.) 212, 197–198 (1966)
Kogan, G.V., Rosovsky, Ya. M., Gvozdev, V.A.: Purification and immunological characteristics of the wild type and mutant forms of 6PGD. Molec. Biologia (Russ.). in press
Lefevre, G.: The relationship between genes and polytene chromosome bands. Ann. Rev. Genet. 8, 51–62 (1974)
Lehninger, A.L.: Biochemistry. New York: Worth publishers 1972
Lindsley, D.L., Grell, E.H.: Genetic variations of Drosophila melanogaster. Carnegie Inst. Wash. Publ. 627 (1968)
Luzzatto, L.: Genetic heterogeneity and pathophysiology of glucose-6-phosphate dehydrogenase deficiency. Brit. J. Haematol. 28, 151–155 (1974)
Olenov, Yu. M.: On the genome organization in Metazoa. Cytologia (Russ.) 16, 403–418 (1974)
Ouchterlony, O.: Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Acta. Path. Microbiol. Scand. 32, 231–240 (1953)
Parr, C.W.: Inhibition of phosphoglucose isomerase. Nature (Lond.) 178, 1401 (1956)
Rawls, J.M., Fristrom, J.W.: A complex genetic locus that controls the first three steps of pyrimidine biosynthesis in Drosophila. Nature (Lond.) 255, 738–740 (1975)
Schalet, A.P., Sankaranarayanan, K.: Evaluation and re-evaluation of genetic radiation hazards in man. I. Interspecific comparison of estimates of mutation rates. Mutation Res. 35, 341–370 (1976)
Schawartz, M., Sofer, W.: Alcohol dehydrogenase-negative mutants in Drosophila: Defects at the structural locus? Genetics 83, 125–136 (1976)
Wahl, G.M., Hughes, S.H., Capecchi, M.D.: Immunological characterization of hypoxanthine-guanine phosphorybosyl transferase mutants of mouse L cells: evidence for mutations at different loci in the HGPRT gene. J. Cell Physiol. 85, 307–320 (1975)
Wasserman, E., Levine, L.: Quantitative micro-complement fixation and its use to study of antigenic structure by specific antigen-antibody inhibition. J. Immunol. 87, 290–295, (1961)
Wright, D.A., Shaw, C.R.: Time of expression of genes controlling specific enzymes in Drosophila embryos. Biochem. Genet. 4, 385–394 (1970)
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Gvozdev, V.A., Gerasimova, T.I., Kogan, G.L. et al. Investigations on the organization of genetic loci in Drosophila melanogaster: lethal mutations affecting 6-phosphogluconate dehydrogenase and their suppression. Molec. Gen. Genet. 153, 191–198 (1977). https://doi.org/10.1007/BF00264735
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DOI: https://doi.org/10.1007/BF00264735