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Linkage of the hordein loci Hor1 and Hor2 with the powdery mildew resistance loci Ml-k and Ml-a on Barley chromosome 5

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Summary

The linkage relationship among the loci Hor1, Hor2, Ml-k and Ml-a on the short arm of chromosome 5 was studied by progeny testing the F2 generation of two crosses. The loci Hor1 and Hor2 code for polypeptides of the storage protein hordein (prolamin) and the loci Ml-k and Ml-a determine the resistance reaction with some powdery mildew fungi cultures. The order of the loci is Ml-k, Hor1, Ml-a, and Hor2, the first named being nearest the centromere. The recombination percentage between Hor1 and Hor2 was determined in the F1 and F2 generations in both crosses, the combined estimate being 7.4±0.9 per cent. The recombination percentage estimated between Ml-k and Hor1 was 4.0±1.3, between Hor1 and Ml-a, 5.3±1.1, and between Ml-a and Hor2, 6.1±1.2. The estimates involving the Ml- loci were all probably a little too high.

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Literature

  • Brandt, A. (1976): Endosperm protein formation during kernel development of wild type and a high-lysine barley mutant. Cereal Chem. 53, 890–901

    Google Scholar 

  • Brandt, A. (1979): Cloning of double stranded DNA coding for hordein polypeptides. Carlsberg Res. Commun. 44, 255–267

    Google Scholar 

  • Chua, N.-H.; Bennoun, P. (1975): Thylakoid membrane polypeptides of Chlamydomones reinhardtii wild type and mutant strains deficient in photosystem II reaction center. Proc. Nat. Acad. Sci. (Wash.) 72, 2175–2179

    Google Scholar 

  • Davis, B.J. (1964): Disc electrophoresis II. Methods and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 404–427

    Google Scholar 

  • Doll, H. (1977): Storage proteins in cereals. In: Genetic diversity in plants (eds.: Muhammed, A.; Aksel, R.; von Borstel, R.C.), pp. 337–347. New York, London: Plenum Publ.

    Google Scholar 

  • Doll, H.; Brown, A.H.D. (1979): Hordein variation in wild (Hordeum spontaneum) and cultivated (H. vulgare) barley. Can. J. Genet. Cytol. 21, 391–404

    Google Scholar 

  • Hiura, U. (1960): Studies on the disease resistance in barley. 4. Genetics of the resistance to powdery mildew. Ber. Ohara Inst. Landwirtsch. Biol. Okayama Univ. 11, 235–300

    Google Scholar 

  • Holder, A.A.; Ingversen, J. (1978): Peptide mapping of the major components of in vitro synthesized barley hordein: Evidence of structural homology. Carlsberg Res. Comm. 43, 177–184

    Google Scholar 

  • Ingversen, J. (1975): Structure and composition of protein bodies from wild-type and high-lysine barley endosperm. Hereditas 81, 69–76

    Google Scholar 

  • Jensen, J. (1979): Coordinator's report. Chromosome 5. Barley Genet. Newsl. 9, 118–120. Colorado State Univ.

    Google Scholar 

  • Karimi, H. (1965): Untersuchungen zur Vererbung von Mehltauresistenz der Braugerste als Beitrag wirksamer Krankheitsbekämpfung durch Resistenzzüchtung. Z. Pflanzenzücht. 53, 205–225

    Google Scholar 

  • Køie, B.; Ingversen, J.; Andersen, A.J.; Doll, H.; Eggum, B.O. (1976): Composition and nutritional quality of barley protein. In: Evaluation of seed protein alterations by mutation breeding, p. 55–61. Vienna: Intern. Atomic Energy Agency

    Google Scholar 

  • Køie, B.; Nielsen, G. (1977): Extraction and separation of hordeins. In: Techniques for the separation of barley and maize seed protein (eds.: Miflin, B.J.; Shewry, P.R.), pp. 25–35. Luxembourg: CEC Eur. 5687e

    Google Scholar 

  • Miflin, B.J.; Shewry, P.R. (1979): The biology and biochemistry of cereal seed prolamins. In: Seed protein improvement in cereals and grain legumes. Vol. 1., pp. 137–157. Vienna: Intern. Atomic Energy Agency

    Google Scholar 

  • Moseman, J.G. (1972): Isogenic barley lines for reaction to Ery-siphe graminis f. sp Hordei. Crop. Sci. 12, 681–682

    Google Scholar 

  • Moseman, J.G.; Macer, R.C.F.; Greeley, L.W. (1965): Genetic studies with cultures of Erysiphe graminis f. sp. hordei virulent on Hordeum spontaneum. Trans. Br. Mycol. Soc. 48, 479–489

    Google Scholar 

  • Netsvetaev, V.P. (1978): Mapping of loci Hrd in chromosome 5 of barley with the help of reciprocal translocations. Biologicheskie Osmovy Ratseonalnogo Ispolzovaniya i Jivotnogo Rastitelnogo Myra, pp. 145–146. Riga (USSR): Zinatne (in Russian, translated by V.P. Netsvetaev)

    Google Scholar 

  • Qram, R.N.; Doll, H.; Køie, B. (1975): Genetics of two storage protein variants in barley. Hereditas 80, 53–58

    Google Scholar 

  • Shewry, P.R.; Pratt, H.M.; Charlton, M.J.; Miflin, B.J. (1977): Two-dimensional separation of the prolamin of normal and high-lysine barley (Hordeum vulgare L.) J. Exp. Bot. 28, 597–606

    Google Scholar 

  • Shewry, P.R.; Hill, J.M.; Pratt, H.M.; Leggatt, M.M.; Miflin, B.J. (1978a): An evaluation of techniques for the extraction of hordein and glutelin from barley seed and a comparison of the protein composition of Bomi and Risø 1508. J. Exp. Bot. 29, 677–692

    Google Scholar 

  • Shewry, P.R.; Pratt, H.M.; Finch, R.A.; Miflin, B.J. (1978b): Genetic analysis of hordein polypeptides from single seeds of barley. Heredity 40, 463–466

    Google Scholar 

  • Solari, R.M.; Favret, E.A. (1971): Polymorphism in endosperm proteins of barley and its genetic control. In: Barley genetics II (ed.: Nilan R.), pp. 23–31. Washington State Univ. Press

  • Sozinov, A.A.; Netsvetaev, V.P.; Grigoryan, E.M.; Obraztsov, I.S. (1979): Mapping of the Hrd loci in barley (Hordeum vulgare). Soviet Genetics 14, 1137–1147 (Translation of Genetica 14, (1978) 1610–1619)

    Google Scholar 

  • Starling, T.M.; Pinda, C.R.; Chen, K.; Moseman, J.G. (1963): Loci of genes conditioning resistance in several barley varieties to race 9 of Erysiphe graminis f. sp. hordei. Crop Sci. 3, 151–154

    Google Scholar 

  • Torp, J.; Jensen, H.P.; Jørgensen, J.H. (1978): Powdery mildew resistance genes in 106 northwest European spring barley varieties. In: Royal Veterinary and Agricultural University, Year-book, pp. 75–102. Copenhagen

  • Wiberg, A. (1974a): Genetical studies of spontaneous sources of resistance to powdery mildew in barley. Hereditas 77, 89–148

    Google Scholar 

  • Wiberg, A. (1974b): Sources of resistance to powdery mildew in barley. Hereditas 78, 1–40

    Google Scholar 

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Communicated by D. von Weltstein

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Jensen, J., Jørgensen, J.H., Jensen, H.P. et al. Linkage of the hordein loci Hor1 and Hor2 with the powdery mildew resistance loci Ml-k and Ml-a on Barley chromosome 5. Theoret. Appl. Genetics 58, 27–31 (1980). https://doi.org/10.1007/BF00264664

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