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A quantitative assay to measure chromosome stability in Schizosaccharomyces pombe

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Summary

The fidelity of mitotic chromosome transmission in Schizosaccharomyces pombe was estimated quantitatively by using cycloheximide resistance as a means to select cells that had undergone chromosome loss or nondisjunction. We aimed to investigate the connection between recombination and mitotic chromosome stability. A number of mutants defective in mitotic recombination such as cdc17-L16, rec59-72, and rec50-25 were tested and in these an approximately ten fold elevation of mitotic haploidization rate was found compared with controls. Our data suggest that recombination is important in controlling the maintenance of chromosomes during mitosis.

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References

  • Egel R (1973) Genes involved in mating-type expression of fission yeast. Mol Gen Genet 122:339–343

    Google Scholar 

  • Gutz H, Heslot H, Leupold U, Loprieno N (1974) Schizosaccharomyces pombe. In: King RC (ed) Handbook of genetics. Plenum Press, New York, pp 395–446

    Google Scholar 

  • Hartwell LH, Smith D (1985) Altered fidelity of mitotic chromosome transmission in cell cycle mutants of S. cerevisiae. Genetics 110:381–395

    CAS  PubMed  Google Scholar 

  • Hartwell LH, Deutcher SK, Wood JS, Garvik B (1982) The fidelity of mitotic chromosome reproduction in S. cerevisiae. Recent Adv Yeast Mol Biol 1:28–38

    Google Scholar 

  • Hieter P, Mann C, Snyder M, Davis RW (1985) Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss. Cell 40:381–392

    Google Scholar 

  • Hirano T, Funahashi S, Kemura T, Yanagida M (1986) Isolation and characterization of S. pombe cut mutants that block nuclear division but not cytokinesis. EMBO J 5:2973–2979

    Google Scholar 

  • Hiraoka Y, Toda T, Yanagida M (1984) The NDA3 gene of fission yeast encodes β-tubulin: A cold-sensitive nda3 mutation reversibly blocks spindle formation and chromosome movement in mitosis. Cell 39:349–358

    Google Scholar 

  • Holliday R (1989) Chromosome error propagation and cancer. Trends Genet 5:42–45

    Google Scholar 

  • Koshland D, Kent JC, Hartwell LH (1985) Genetic analysis of mitotic transmission of minichromosomes. Cell 40:393–403

    Google Scholar 

  • Lea DE, Coulson CA (1949) The distribution of the numbers of the mutants in bacterial populations. J Genet 49:264–285

    Google Scholar 

  • Leupold U (1950) Die Verebung von Homothallie und Heterothallie bei Schizosaccharomyces pombe. CR Lab Carlsberg Ser Physiol 24:381–480

    Google Scholar 

  • Meeks-Wagner D, Wood JS, Garvik B, Hartwell LH (1986) Isolation of two genes that effect mitotic chromosome transmission in S. cerevisiae. Cell 44:53–63

    Google Scholar 

  • Nasmyth KA (1977) Temperature-sensitive lethal mutants in the structural gene for DNA ligase in the yeast Schizosaccharomyces pombe. Cell 12:1109–1120

    Google Scholar 

  • Ohkura H, Adachi Y, Kinoshita N, Niwa O, Toda T, Yanagida M (1988) Cold-sensitive and caffeine-supersensitive mutants of the S. pombe dis genes implicated in sister chromatid separation during mitosis. EMBO J 7:1465–1473

    Google Scholar 

  • Sipiczki M, Grossenbacher-Grunder A-M, Bodi Zs (1989) The effect of cdc17-L16 on recombination. Mol Gen Genet 220:307–313

    Google Scholar 

  • Surosky RT, Tye B (1988) Meiotic disjunction of homologs in Saccharomyces cerevisiae is directed by pairing and recombination of chromosome arms but not by pairing of centromeres. Genetics 119:273–287

    Google Scholar 

  • Toda T, Yamamoto M, Yanagida M (1981) Sequential alterations in nuclear chromatin during mitosis of fission yeast Schizosaccharomyces pombe. Video fluorescence microscopy of synchronously growing wild type and cold-sensitive cdc mutants by using a DNA-binding fluorescent probe. J Cell Sci 52:271–278

    Google Scholar 

  • Uemura T, Ohkura H, Adachi Y, Marino K, Shiozaki K, Yanagida M (1987) DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe. Cell 50:917–925

    Google Scholar 

  • Whittaker SG, Rockmill BM, Bleachel AE, Maloney DH, Resnick MA, Fogel S (1988) The detection of mitotic and meiotic aneuploidy in yeast using a gene dosage selection system. Mol Gen Genet 215:10–18

    Google Scholar 

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Author notes

  1. Zsuzsa Bodi

    Present address: Department of Plant Sciences, University of Oxford, OX1 3RB, Oxford, UK

Authors and Affiliations

  1. Department of Genetics, Lajos Kossuth University, H-4010, Debrecen, Hungary

    Zsuzsa Bodi & Annelis Gysler-Junker

  2. Institute of General Microbiology, University of Bern, CH-3012, Bern, Switzerland

    Jurg Kohli

Authors
  1. Zsuzsa Bodi
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  2. Annelis Gysler-Junker
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  3. Jurg Kohli
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Communicated by J. Gajewski

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Bodi, Z., Gysler-Junker, A. & Kohli, J. A quantitative assay to measure chromosome stability in Schizosaccharomyces pombe . Molec. Gen. Genet. 229, 77–80 (1991). https://doi.org/10.1007/BF00264215

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  • DOI: https://doi.org/10.1007/BF00264215

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