Summary
Mitochondrial DNA (mtDNA) of the Black Mexican sweet line of maize isolated from tissue cultured cell suspension cultures and young seedlings was examined. Restriction fragments generated by two endonucleases were comparatively analyzed by visualization of ethidium bromide stained gels as well as by membrane hybridization with nick-translated DNA probes of plasmid-like S1 and S2 DNA. Although no major molecular alterations were seen in tissue cultured cells, the samples were clearly not identical. The variation was mainly in the stoichiometry of several restriction fragments. Hybridization analyses with S1 and S2 probes show no evidence of molecular rearrangement in this part of the genome in tissue cultured cells. Minor variations in restriction patterns could reflect alterations in frequency of circular mtDNA molecules, perhaps related to nuclear alterations during the extended period of culture.
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References
Borck KS, Walbott V (1982) Comparisons of the restriction endonuclease patterns of mitochondrial DNA from normal and male-sterile cytoplasms of Zea mays L. Genetics 102:109–128
Brar DS, Rambold S, Gamburg O, Constable F (1979) Tissue culture of corn and sorghum. Z Pflanzenphysiol 95:377–388
Brettell RIS, Conde MF, Pring DR (1982) Analysis of mitochondrial DNA from four different maize lines obtained from a tissue culture carrying Texas cytoplasm. Maize Genet Coop Newslett 56:13–14
Chourey PS, Kemble RJ (1982) Transposition event in tissue cultured cells of maize. In: 5th Int Congr Plant Tissue Cell Culture. Tokyo, Japan, pp 425–426
Chourey PS, Zurawski DB (1981) Callus formation from protoplasts of a maize cell culture. Theor Appl Genet 59:341–344
De Francesco L, Attardi G, Croce CM (1980) Uniparental propagation of mitochondrial DNA in mouse-human hybrids. Proc Natl Acad Sci USA 77:4079–4083
Freeling M, Woodman JC, Cheng DSK (1976) Developmental potentials of maize tissue cultures. Maydica 21:97–112
Gengenbach BG, Connelly JA, Pring DR, Conde MF (1981) Mitochondrial DNA variation in maize plants regenerated during tissue culture selection. Theor Appl Genet 59:161–167
Kemble RJ, Gunn RE, Flavell RB (1980) Classification of normal and male-sterile cytoplasms in maize. 2. Electrophoretic analysis of DNA species in mitochondria. Genetics 95:451–458
Kemble RJ, Mans RJ (1983) Examination of the mitochondrial genome of revertant progeny from S cms maize with cloned S-1 and S-2 hybridization probes. J Mol Appl Genet 2:161–171
Kim BD, Mans RJ, Conde MF, Pring DR, Levings CS III (1982) Physical mapping of homologous segments of mitochondrial episoms from S male-sterile maize. Plasmid 7:1–14
Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214
Laughnan JR, Gabay-Laughnan S, Carlson JE (1981) Characteristics of cms-S reversion to male-fertility in maize. Stadtler Symp 13:93–114
Levings CS III, Kim BD, Pring DR, Conde MF, Mans RJ, Laughnan JR, Gabay-Laughnan SJ (1980) Cytoplasmic reversion of cms-S in maize: association with a transpositional event. Science 209:1021–1023
Lonsdale DM, Thompson RD, Hodge TP (1981) The integrated forms of the S1 and S2 DNA elements of maize male-sterile mitochondrial DNA are flanked by a large repeated sequence. Nucleic Acids Res 9:3657–3669
McNay JW, Pring DR, Lonsdale DM (1983) Polymorphism of mitochondrial DNA “S” regions among normal cytoplasms of maize. Plant Mol Biol 2:177–187
Potrykus I, Harms CT, Lorz H (1979) Callus formation from cell culture protoplasts of corn (Zea mays L.). Theor Appl Genet 54:209–214
Pring DR, Levings CS III (1978) Heterogeneity of maize cytoplasmic genomes among male-sterile cytoplasms. Genetics 89:121–136
Pring DR, Levings CS III, Hu WWL, Timothy DH (1977) Unique DNA associated with mitochondria in the “S”-type cytoplasm of male-sterile maize. Proc Natl Acad Sci USA 74:2904–2908
Rigby PWJ, Dieckman M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick-translation with polymerase I. J Mol Biol 113:237–251
Shepard JF, Bidney D, Shahin E (1980) Potato protoplasts in crop improvement. Science 208:17–24
Sheridan WF (1975) Growth of corn cells in culture. J Cell Biol 67:396 a
Sheridan WF (1982) Black Mexican sweet corn: its use for tissue culture. In: Sheridan WF (ed) Maize for biological research. Plant Molecular Biology Ass, Grand Forks, North Dakota, pp 385–386
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Spruill WM Jr, Levings CS III, Sederhoff RR (1980) Recombinant DNA analysis indicates that the multiple chromosomes of maize mitochondria contain different sequences. Dev Genet 1:363–378
Thompson RD, Kemble RJ, Flavell RB (1980) Variation in mitochondrial DNA organization between normal and male-sterile cytoplasms. Nucleic Acids Res 8:1999–2008
Yang RCA, Lis J, Wu R (1979) Elution of DNA from agarose gels after electrophoresis. In: Wu R (ed) Methods in enzymology, vol 68. Academic Press, London New York, pp 176–182
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Communicated by P. L. Pfahler
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McNay, J.W., Chourey, P.S. & Pring, D.R. Molecular analysis of genomic stability of mitochondrial DNA in tissue cultured cells of maize. Theoret. Appl. Genetics 67, 433–437 (1984). https://doi.org/10.1007/BF00263407
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DOI: https://doi.org/10.1007/BF00263407