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Effect of hyperthermia and doxorubicin on nucleoid sedimentation and poly (ADP-ribose) Polymerase activity in L1210 cells

  • Original Articles
  • Doxorubicin, Hyperthermia, L1210 Cells
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Summary

We report on the individual and combined effects of doxorubicin (DOX) and hyperthermia (HYP) on nucleoid sedimentation and poly (ADP-ribose) polymerase (PARP) activity of L1210 cells. The effects of HYP and DOX on nucleoid sedimentation (increased sedimentation) were similar and correlated with cell viability. No correlation of PARP activity with cell toxicity was evident; the activity of PARP was inhibited by HYP (42°C; 1–3 h) and stimulated by DOX (1–10 μM; 30 min). The HYP-induced inhibition of PARP was actually ameliorated by simultaneous exposure to DOX. Although separate studies have previously suggested that chromatin alterations or the inhibition of PARP might play a role in the effect of HYP, the correlation of nucleoid changes (rather than PARP activity) with cell viability emphasizes the contribution of the former. Furthermore, the results suggest that the nucleoid technique may prove useful in screening potential treatment modalities.

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Authors and Affiliations

  1. Department of Medical Oncology, Fox Chase Cancer Center, Central and Shelmire Avenues, 19111, Philadelphia, PA, USA

    J. Patrick Daugherty

  2. Comprehensive Cancer Center, University of Alabama in Birmingham, 35294, Birmingham, AL, USA

    Thomas A. Simpson Jr.

  3. Northwest Alabama Cancer Center, 203 West Avalon Avenue, Suite 270, 35661, Muscle Shoals, AL, USA

    Dail W. Mullins Jr.

Authors
  1. J. Patrick Daugherty
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  2. Thomas A. Simpson Jr.
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  3. Dail W. Mullins Jr.
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Daugherty, J.P., Simpson, T.A. & Mullins, D.W. Effect of hyperthermia and doxorubicin on nucleoid sedimentation and poly (ADP-ribose) Polymerase activity in L1210 cells. Cancer Chemother. Pharmacol. 21, 229–232 (1988). https://doi.org/10.1007/BF00262775

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  • DOI: https://doi.org/10.1007/BF00262775

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