Summary
In 18 control subjects and in 41 Type 1 (insulin-dependent) diabetic patients (13 with normal proteinuria, group A; 15 with microproteinuria, group B; and 13 with clinical proteinuria, group C), mean blood glucose, glycosylated haemoglobin and non-enzymatic glycosylated serum and urinary proteins, expressed as 5-hydroxymethylfurfural (5-HMF), were measured. In each group of diabetic patients, the levels of mean daily blood glucose, glycosylated haemoglobin and serum 5-HMF/mg protein were higher than in the control subjects. The urinary 5-HMF/mg proteinuria and the urinary/serum 5-HMF concentration ratio values were raised in group A and reduced in groups B and C. Moreover, they showed a negative correlation with 24-h urinary protein excretion in the control subjects and in each group of diabetic patients. The urinary 5-HMF/day in groups A, B and C was greater than in the control subjects. The urinary 5-HMF/day did not correlate with the mean daily blood glucose levels and, only in group A, did it correlate with serum 5-HMF and glycosylated haemoglobin. This suggests that, in this group, functional factors result in the increased renal elimination of 5-HMF and, therefore, of non-enzymatically glycosylated proteins. However, in the other groups of patients, this elimination depends on the degree of proteinuria.
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Viberti GC, Pickup JC, Jarrett RJ, Keen H (1979) Effect of control of blood glucose on urinary excretion of albumin and B2-microglobulin in insulin-dependent diabetes. N Engl J Med 300: 638–641
Viberti GC, Mackintosh D, Bilous RW, Pickup JC, Keen H (1982) Proteinuria in diabetes mellitus: role of spontaneous and experimental variation of glycaemia. Kidney Int 21: 715–720
Gragnoli G, Signorini AM, Tanganelli I, Tarli P, Paoli C (1981) Non-enzymatic glycosylation of serum proteins in diabetes Type 1 before and during intensive insulin treatment. Rass Med Intern 2 (Suppl 4): 169 (Abstract)
Williams SK, Devenny JJ, Bitensky MW (1981) Micropinocitic ingestion of glycosylated albumin by isolated microvessels: possible role in pathogenesis of diabetic microangiopathy. Proc Natl Acad Sci 78: 2393–2397
McVerry BA, Fisher C, Hopp A, Huehns ER (1980) Production of pseudodiabetic renal glomerular changes in mice after repeated injections of glycosylated proteins. Lancet 1: 738–740
Cohen MP, Urdanivia E, Surma M, Wu VY (1980) Increased glycosylation of glomerular basement membrane collagen in diabetes. Biochem Biophys Res Commun 95: 756–769
Viberti GC, Mackintosh D, Keen H (1983) Determinants of the penetration of proteins through the glomerular barrier in insulindependent diabetes mellitus. Diabetes 32 (Suppl 2): 92–95
Anonymous (1959) New weight standards for men and women. Stat Bull Metropol Life Ins Co 40
Trinder P (1969) Determination of glucose in blood using glucoseoxidase with an alternative oxygen acceptor. Ann Clin Biochem 6: 24–27
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal Biochem 72: 248–254
Gabbay KH, Hasty K, Breslow JL, Ellison RC, Bunn HF, Gallop PM (1977) Glycosylated haemoglobins and long-term blood glucose control in diabetes mellitus. J Clin Endocrinol Metab 44: 859–864
Kennedy AL, Mehl TD, Merimee TJ (1980) Nonenzymatically glycosylated serum protein: spurious elevation due to free glucose in serum. Diabetes 29: 413–415
Yue DK, Morris K, McLennan S, Turtle JR (1980) Glycosylation of plasma protein and its relation to glycosylated haemoglobin in diabetes. Diabetes 29: 296–300
Gragnoli G, Tanganelli I, Signorini AM, Tarli P, Paoli C (1982) Aspects of non-enzymatic glycosylation of serum proteins in relation to different glucose loads. Diabetologia 22: 495–496
Dolhofer R, Wieland OM (1981) Improvement of the thiobarbituric acid assay for serum glycosyl protein determination. Clin Chim Acta 112: 197–204
Mednick ML (1962) The acid base catalyzed conversion of aldohexose into 5-(hydroxymethyl)-2 furfural. J Org Chem 27: 398–403
Bunn HF (1981) Nonenzymatic glycosylation of protein. Relevance to diabetes. Am J Med 70: 325–330
Candiano G, Ghiggeri GM, Delfino G, Queirolo C (1983) Molecular basis for cationization of glycosylalbumin: importance of three-dimensional structure of albumin. Diabetologia 25: 145 (Abstract)
Westberg NG, Michael AF (1973) Human glomerular basement membrane: chemical composition in diabetes mellitus. Acta Med Scand 194: 39–47
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Gragnoli, G., Signorini, A.M. & Tanganelli, I. Non-enzymatic glycosylation of urinary proteins in Type 1 (insulin-dependent) diabetes: correlation with metabolic control and the degree of proteinuria. Diabetologia 26, 411–414 (1984). https://doi.org/10.1007/BF00262211
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DOI: https://doi.org/10.1007/BF00262211